Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00008411-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00008411-M03, RRID:AB_1145446
- Product name
- Mouse Monoclonal EEA1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. EEA1 - early endosome antigen 1, 162kD (2G2)
- Reactivity
- Human, Mouse, Hamster
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Flipping the Switch on Clathrin-Mediated Endocytosis using Thermally Responsive Protein Microdomains.
Pastuszka MK, Okamoto CT, Hamm-Alvarez SF, MacKay JA
Advanced functional materials 2014 Sep 10;24(34):5340-5347
Advanced functional materials 2014 Sep 10;24(34):5340-5347
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of EEA1 expression in A-431 (Cat # L015V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: EEA1 Antibody (2G2) [H00008411-M03] - Detection limit for recombinant GST tagged EEA1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: EEA1 Antibody (2G2) [H00008411-M03] - Analysis of monoclonal antibody to EEA1 on HeLa cell. Antibody concentration 10 ug/ml