PA5-29013

antibody from Invitrogen Antibodies

Targeting: EEA1 ZFYVE2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29013

Provider

Invitrogen Antibodies

Product name

EEA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Recommended positive controls: HepG2, A375, mouse brain, PC-12, Rat-2, A549, MNT-1. Predicted reactivity: Mouse (100%), Rat (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat, Hamster

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of EEA1 using A) 30 µg HepG2 whole cell lysate and B) 30 µg A375 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an EEA1 polyclonal antibody (Product # PA5-29013) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using EEA1 Polyclonal Antibody (Product # PA5-29013). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 5% SDS PAGE. EEA1 Polyclonal Antibody (Product # PA5-29013) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EEA1 Polyclonal Antibody detects EEA1 protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract. B. 30 µg A375 whole cell lysate/extract.5% SDS-PAGE. EEA1 Polyclonal Antibody (Product # PA5-29013) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EEA1 Polyclonal Antibody detects EEA1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract. B. 30 µg Rat2 whole cell lysate/extract.5% SDS-PAGE. EEA1 Polyclonal Antibody (Product # PA5-29013) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EEA1 in methanol-fixed HeLa cells using an EEA1 polyclonal antibody (Product # PA5-29013) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of EEA1 was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: EEA1 Polyclonal Antibody (Product # PA5-29013) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EEA1 Polyclonal Antibody detects EEA1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. EEA1 Polyclonal Antibody (Product # PA5-29013) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EEA1 Polyclonal Antibody detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EEA1 Polyclonal Antibody (Product # PA5-29013) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EEA1 Polyclonal Antibody detects EEA1 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EEA1 Polyclonal Antibody (Product # PA5-29013) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.