MA5-17226

antibody from Invitrogen Antibodies

Targeting: AURKB Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA5-17226

Provider

Invitrogen Antibodies

Product name

Anti-Aurora B Monoclonal Antibody (AF7B11)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is A431 cells.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

AF7B11

Vial size

100 µL

Concentration

2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Aurora B using anti-Aurora B monoclonal antibody (Product # MA5-17226) on (Lane 1) A431 Cell lysate, (Lane 2) HT29 Cell lysate, (Lane 3) SK-N-MC Cell lysate, (Lane 4) Jurket Cell lysate, (Lane 5) SK-BR3 Cell lysate and (Lane 6) PC12 Cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Aurora B Monoclonal Antibody (Product # MA5-17226) and a 39kDa band corresponding to Aurora B was observed across cell lines tested and increased upon Nocodazole treatment. Whole cell extracts (30 µg lysate) of NTERA-2 (Lane 1), T47-D (Lane 2), A-549 (Lane 3), HEK-293 (Lane 4), HeLa (Lane 5), HeLa treated with Nocodazole (200ng/mL for 12 hr) (Lane 6) and THP-1 (Lane 6) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2ug/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).