TA319253

antibody from OriGene

Targeting: AURKB Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319253

Provider

OriGene

Product name

Rabbit polyclonal Aurora B pT232 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal Aurora B pT232 antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

AURKB

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot shows detection of Aurora B protein at 39 kDa (predicted band size). All lanes : Aurora B (phospho T232) antibody diluted 1:500. Lane 1 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs). Lane 2 : Extract from COS7 cells treated with Nocodazole (1ug/ml, 16 hrs) and with the phosphopeptide immunogen.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of Rabbit Anti-AuroraB pT232 Antibody. Tissue: human placenta pH9 (A) at 20x and 40x. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AuroraB pT232 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AuroraB pT232 is cytoplasmic. Staining: AuroraB pT232 as precipitated brown signal with hematoxylin purple nuclear counterstain.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry of Rabbit Anti-AuroraB pT232 Antibody. Tissue: human intestine pH9 (A) at 20x and 40x. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AuroraB pT232 antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AuroraB pT232 is cytoplasmic. Staining: AuroraB pT232 as precipitated brown signal with hematoxylin purple nuclear counterstain.

Validation comment

IHC