GTX85607

antibody from GeneTex

Targeting: AURKB Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX85607

Provider

GeneTex

Proper citation

GeneTex Cat#GTX85607, RRID:AB_10722499

Product name

Aurora B (phospho Thr232) antibody

Antibody type

Polyclonal

Reactivity

Human, Simian

Host

Rabbit

References

Submitted references

Bioisosteric replacement of an acylureido moiety attached to an indolin-2-one scaffold with a malonamido or a 2/4-pyridinoylamido moiety produces a selectively potent Aurora-B inhibitor.

Wang HC, Jagtap AD, Chang PT, Liu JR, Liu CP, Tseng HW, Chen GS, Chern JW
European journal of medicinal chemistry 2014 Sep 12;84:312-34

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Novel acylureidoindolin-2-one derivatives as dual Aurora B/FLT3 inhibitors for the treatment of acute myeloid leukemia.

Jagtap AD, Chang PT, Liu JR, Wang HC, Kondekar NB, Shen LJ, Tseng HW, Chen GS, Chern JW
European journal of medicinal chemistry 2014 Oct 6;85:268-88

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western Blot shows detection of Aurora B protein at 39 kD (predicted band size). All lanes: Aurora B (phospho T232) antibody diluted 1:500. Lane 1: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) Lane 2: Extract from COS7 cells treated with Nocodazole (1 ug/ml, 16 hrs) and with the phosphopeptide immunogen

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Human Tonsil (formalin-fixed, paraffin-embedded) stained with AURKB antibody (GTX85607) at 2.5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody LS-D1, alkaline phosphatase-streptavidin and chromogen.