P00762
antibody from Boster Biological Technology
Targeting: AURKB
Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- P00762 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Phospho-Aurora B pT232 Aurkb Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Aurora B/Aurkb detection. Host: Rabbit.Size: 100μg. Tested applications: IF. Reactive species: Human. Aurora B/Aurkb information: Molecular Weight: 39384 MW; Subcellular Localization: Nucleus . Chromosome . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Midbody . Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalized with gamma tubulin in the mid-body (By similarity). Proper localization of the active, Thr-237-phosphorylated form during metaphase may be dependent upon interaction with SPDYC. Colocalized with SIRT2 during cytokinesis with the midbody (By similarity); Tissue Specificity: Expressed in testis, intestine and spleen. All of them are tissues that contain a large number of proliferating cells. Expressed during S phase, in a cell-cycle-dependent fashion.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 100μg
- Concentration
- 0.87 mg/mL by UV absorbance at 280 nm
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Aurora B expression in Extract from COS7 cells treated with Nocodazole (lane 1) and extract from COS7 cells treated with Nocodazole and with the phosphopeptide immunogen (lane 2). Aurora B at 39KD was detected using rabbit anti-Aurora B pT232 Antigen Affinity purified polyclonal antibody (Catalog # P00762) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image