711154

antibody from Invitrogen Antibodies

Targeting: AURKB Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711154

Provider

Invitrogen Antibodies

Product name

Aurora B Recombinant Polyclonal Antibody (18HCLC)

Antibody type

Polyclonal

Antigen

Other

Description

This antibody is predicted to react with Monkey, Horse, Cat and Bovine

Antibody clone number

18HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of T-47D (Lane 1), K-562 (Lane 2), NTERA-2 (Lane 3), A-431 (Lane 4) and HeLa (Lane 5). The blots were probed with Anti-Aurora B Kinase Recombinant Rabbit Polyclonal Antibody (Product # 711154, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 40 kDa band corresponding to Aurora B Kinase was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).