PA5-17632

antibody from Invitrogen Antibodies

Targeting: AURKB Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17632

Provider

Invitrogen Antibodies

Product name

Aurora B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

27 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Aurora B Polyclonal Antibody (Product # PA5-17632) and a 39kDa band corresponding to Aurora B was observed across cell lines tested and increased upon Nocodazole treatment. Whole cell extracts (30 µg lysate) of NTERA-2 (Lane 1), T47-D (Lane 2), A-549 (Lane 3), HEK-293 (Lane 4), HeLa (Lane 5), HeLa treated with Nocodazole (200ng/mL for 12 hr) (Lane 6) and THP-1 (Lane 7) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Aurora B was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with Aurora B Monoclonal Antibody (Product # PA5-17632) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Aurora B/AIM1 in Hela cells using a Aurora B/AIM1 polyclonal antibody (Product # PA5-17632) (blue) compared to a nonspecifc negative control antibody (red).