NBP1-74027-100ul

antibody from Novus Biologicals

Targeting: AURKB Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-74027-100ul

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-74027-100ul, RRID:AB_11137267

Product name

Mouse Monoclonal Aurora B Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ul

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Aurora B Antibody (2B1) [NBP1-74027] - The recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with antihuman Aurora kinase B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Aurora kinase A Lane 2.: Recombinant Human Aurora kinase B

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: Aurora B Antibody (2B1) [NBP1-74027] - HeLa cells stained with Hoechst 3342 (Blue) for chromatin staining and monoclonal anti-human Aurora kinase B antibody (1:2000) with Texas Red (Red).

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of AURKB in LNCaP cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of AURKB in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Aurora B Antibody (2B1) [NBP1-74027] - Analysis of Aurora B in LNCap cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).