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LearnAF4006
antibody from R&D Systems
Targeting: AURKB
Aik2, AIM-1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF4006 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Aurora B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse Aurora B in Western blots.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q96GD4- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Generation of human induced pluripotent stem cells using epigenetic regulators reveals a germ cell-like identity in partially reprogrammed colonies.
Goyal A, Chavez SL, Reijo Pera RA
PloS one 2013;8(12):e82838
PloS one 2013;8(12):e82838
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Aurora B by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human Aurora B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4006) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Aurora B at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
