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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA501961 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501961, RRID:AB_11124453
- Product name
- SPR mouse monoclonal antibody, clone OTI3A9 (formerly 3A9)
- Antibody type
- Monoclonal
- Description
- SPR mouse monoclonal antibody, clone OTI3A9 (formerly 3A9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SPR
- Isotype
- IgG
- Antibody clone number
- OTI3A9
- Vial size
- 100 µl
- Concentration
- 0.76 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (RC205679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SPR mouse monoclonal antibody (TA501961) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR(RC205679).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC205679 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SPR antibody(TA501961), and then analyzed by flow cytometry.
- Validation comment
- FC
