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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- H00006697-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006697-M01, RRID:AB_1147096
- Product name
- Mouse Monoclonal SPR Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SPR - sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) (4F2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SPR Antibody (4F2) [H00006697-M01] - Analysis of SPR expression in transfected 293T cell line by SPR monoclonal antibody (M01), clone 4F2.Lane 1: SPR transfected lysate(28 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: SPR Antibody (4F2) [H00006697-M01] - Detection limit for recombinant GST tagged SPR is 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SPR Antibody (4F2) [H00006697-M01] - Analysis of SPR transfected lysate using anti-SPR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SPR MaxPab rabbit polyclonal antibody.
