A301-641A-T

antibody from Invitrogen Antibodies

Targeting: POLL

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-641A-T

Provider

Invitrogen Antibodies

Product name

Pol Lambda Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse Pol Lambda by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Pol Lambda (Cat. No. A301-641A Lot2) used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse Pol Lambda by immunohistochemistry. Samples: FFPE section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-Pol Lambda (Cat. No. A301-641A-T Lot1) used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pol Lambda by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pol Lambda (Cat. No. A301-641A Lot2) used at a dilution of 1:1,000 (1 µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody A301-641A (lot A301-641A-2) used for IP at 6 µg per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody A301-640A. For blotting immunoprecipitated Pol Lambda, A301-640A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.