NB100-1568

antibody from Novus Biologicals

Targeting: GNL3 C77032, E2IG3, MGC800, NS

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1568

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1568, RRID:AB_2110090

Product name

Rabbit Polyclonal Nucleostemin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Abundant nucleostemin expression supports the undifferentiated properties of germ cell tumors.

Uema N, Ooshio T, Harada K, Naito M, Naka K, Hoshii T, Tadokoro Y, Ohta K, Ali MA, Katano M, Soga T, Nakanuma Y, Okuda A, Hirao A
The American journal of pathology 2013 Aug;183(2):592-603

---

Maintenance of tumor initiating cells of defined genetic composition by nucleostemin.

Okamoto N, Yasukawa M, Nguyen C, Kasim V, Maida Y, Possemato R, Shibata T, Ligon KL, Fukami K, Hahn WC, Masutomi K
Proceedings of the National Academy of Sciences of the United States of America 2011 Dec 20;108(51):20388-93

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Nucleostemin Antibody [NB100-1568] - Detection of Human and Mouse GNL3 on HeLa whole cell lysate using NB100-1568. GNL3 was also immunoprecipitated using rabbit anti-GNL3 antibodies NB100-1569 and another GNL3 Ab.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Nucleostemin Antibody [NB100-1568] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-GNL3 used at a dilution of 1:200 (1ug/ml). Detection: DAB