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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27131 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX5 Polyclonal Antibody (Product # PA5-27131). Sample (30 µg of whole cell lysate). Lane A: H1299 . 7.5% SDS PAGE. DDX5 antibody . DDX5 Polyclonal Antibody (Product # PA5-27131) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDX5 in paraformaldehyde-fixed A431 cells using a DDX5 polyclonal antibody (Product # PA5-27131) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded lung SCC xenograft, using DDX5 (Product # PA5-27131) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
