M04133

antibody from Boster Biological Technology

Targeting: MARCKSL1 F52, MacMARCKS, MLP, MLP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

M04133

Provider

Boster Biological Technology

Product name

Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody

Antibody type

Polyclonal

Description

Anti-MARCKS (Myristoylated Alanine Rich C Kinase Substrate) Antibody tested in WB, IF, ICC, reactive to Human

Reactivity

Human

Host

Rabbit

Vial size

100ul

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Storage for short term at 4℃ recommended, for longer term at -20℃, minimize freeze/thaw cycles

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of cell line lysates probed simultaneously with rabbit pAb to MARCKS, M04133, dilution 1:1,000, in green and mouse mAb to GAPDH, dilution 1:5,000 in red: [1] protein standard (red), [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, [7] C6 cells. The strong band at ~80kDa corresponds to MARCKS protein, detected only in the proteins of human origin. Slight reactivity is observed in the monkey cells [5], but no reactivity is seen on rodent cells. GAPDH antibody used as a loading control revealing a single band at ~37kDa in all preparations.

Additional image