AP09318PU-N

antibody from Acris Antibodies GmbH

Targeting: PPP1R13L IASPP, RAI

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09318PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09318PU-N, RRID:AB_2035654

Product name

anti PPP1R13L (780-797)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 780-797 of Human iASPP (isoform 1) protein

Reactivity

Human, Mouse, Canine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.19 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using AP09318PU-N iASPP antibody shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1/1,500 in 5% LOTTO/PBS overnight at 4°C. The membrane was washed and reacted with a 1/10,000 dilution of IRDye(TM)800 conjugated Goat anti-Rabbit IgG [H&L] for 45 min at RT (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP09318PU-N iASPP antibody staining of Formalin-Fixed, Paraffin Embedded Human heart tissue. This antibody shows strong cytoplasmic and membranous staining of myocytes. Brown color indicates presence of protein, blue color shows cell nuclei.