Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-29153 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMYD5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SMYD5 Polyclonal Antibody (Product # PA5-29153). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. 10% SDS PAGE. SMYD5 Polyclonal Antibody (Product # PA5-29153) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using SMYD5 (Product # PA5-29153) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SMYD5 Polyclonal Antibody detects SMYD5 protein at cytoplasm and nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. SMYD5 Polyclonal Antibody (Product # PA5-29153) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
