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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- GTX629445 - Provider product page
- Provider
- GeneTex
- Product name
- HMGB1 antibody [GT427]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
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- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT427] detects 0 protein by Western blot analysis.A. 30 ?g SK-N-SH whole cell lysate/extractB. 30 ?g IMR32 whole cell lysate/extractC. 30 ?g SK-N-AS whole cell lysate/extract12 % SDS-PAGEHMGB1 antibody [GT427] (GTX629445) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT427] detects HMGB1 protein by western blot analysis. Mouse tissue extracts and Rat tissue extracts (50 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT427] (GTX629445) diluted at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 £gm.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.Red: MAP2, stained by MAP2 antibody (GTX133109) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT427] detects HMGB1 protein at nucleus by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1 protein stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:500.Red: GFAP, a glia cell marker, stained by GFAP antibody (GTX27260) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT427] detects HMGB1 Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: HMGB1 stained by HMGB1 antibody [GT427] (GTX629445) diluted at 1:250.Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
