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- Product number
- MA1-20338 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGB1 Monoclonal Antibody (HAP46.5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: MCF7. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HAP46.5
- Vial size
- 100 µL
- Concentration
- 1.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Greater high-mobility group box 1 in male compared with female spontaneously hypertensive rats worsens renal ischemia-reperfusion injury.
Mohamed R, Rafikova O, O'Connor PM, Sullivan JC
Clinical science (London, England : 1979) 2020 Jul 17;134(13):1751-1762
Clinical science (London, England : 1979) 2020 Jul 17;134(13):1751-1762
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in (lane 1) mouse kidney (lane 2) HeLa (lane 3) HeLa nuclear (lane 4) Jurkat (lane 5) HEK-293T (lane 6) MDCK (lane 7) PC12 (lane 8) NIH-3T3 (lane 9) P19 lysates. Samples were separated on SDS-PAGE and probed with a HMGB1 monoclonal antibody (Product # MA1-20338) at a dilution of 1 µg/mL. The antibody was developed using a Goat Anti-mouse IgG-Peroxidase and a chemiluminescent substrate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of RAW 264.7 (Lane 1), THP-1 (Lane 2), NIH/3T3 (Lane 3), MCF7 (Lane 4) and SH-SY5Y (Lane 5). The blot was probed with Anti-HMGB1 Monoclonal Antibody (Product # MA1-20338, 1 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 29 kDa band corresponding to HMGB1 was observed across the cell lines tested.
