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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- GTX628899 - Provider product page
- Provider
- GeneTex
- Product name
- HMGB1 antibody [GT427]
- Antibody type
- Monoclonal
- Host
- Mouse
- Vial size
- 100 µl
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Strep Tag antibody [GT517] detects strep protein by Western blot analysis.A. 30 µg 293T whole cell lysate/extractB. 30 µg whole cell lysate/extract of strep-tagged protein expressing 293T cells12 % SDS-PAGEStrep Tag antibody [GT517] (GTX628899) dilution: 1:5000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
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- Experimental details
- Strep-tag antibody detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep-tag antibody (GTX628899) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.
