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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AM32945AF-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti HMGB1
- Antibody type
- Monoclonal
- Antigen
- Full-length recombinant Human HMGB1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- IMG19N10B7
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis using HMGB1 antibody: A) Full-length Human HMGB1 protein, B) Human Jurkat cell lysate and C) Mouse NIH 3T3 cell lysate were probed with HMGB1 antibody at 2 µg/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, decalcified, paraffin embedded tissue sections from the paws of mice with collagen-induced arthritis using the HMGB1 antibody. The antibody was used at 5 µg/ml. Data courtesy of Dr Ulf Andersson, Karolinska Institute.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Intracellular Flow Cytometric analysis using HMGB1 antibody: Human Jurkat cells were probed using 0.5 µg of HMGB1 antibody (red) and 0.5 µg of isotype control antibody (green). APC secondary was used for this test.
