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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- TA337101 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal HMGB1/HMG-1 Antibody (1F3)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal HMGB1/HMG-1 Antibody (1F3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HMGB1
- Isotype
- IgG
- Antibody clone number
- 1F3
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: HMGB1 Antibody (1F3) [TA337101] - Blot of crude HeLa cell extract. HMGB1 runs at an apparent molecular weight of 25kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HMGB1 Antibody (1F3) [TA337101] - HeLa cells grown in tissue culture and stained with TA337101 (red), chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). TA337101 reveals strong nuclear stainin
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: HMGB1 Antibody (1F3) [TA337101] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HMGB1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
- Validation comment
- FC
