Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- MAB1690-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human HMGB1/HMG-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human HMGB1/HMG-1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P09429
- Isotype
- IgG
- Antibody clone number
- 115603
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lack of IL-17 Receptor A signaling aggravates lymphoproliferation in C57BL/6 lpr mice.
Anti-high mobility group box-1 (HMGB1) antibody attenuates delayed cerebral vasospasm and brain injury after subarachnoid hemorrhage in rats.
Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH.
Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology.
IL-1α released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts.
Measles virus causes immunogenic cell death in human melanoma.
Human papillomavirus type 8 interferes with a novel C/EBPβ-mediated mechanism of keratinocyte CCL20 chemokine expression and Langerhans cell migration.
Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis.
Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients.
Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease.
Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
Potential role of high-mobility group box 1 in cystic fibrosis airway disease.
E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release.
Pivotal advance: inhibition of HMGB1 nuclear translocation as a mechanism for the anti-rheumatic effects of gold sodium thiomalate.
TLR4 enhances TGF-beta signaling and hepatic fibrosis.
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Corneth OBJ, Schaper F, Luk F, Asmawidjaja PS, Mus AMC, Horst G, Heeringa P, Hendriks RW, Westra J, Lubberts E
Scientific reports 2019 Mar 11;9(1):4032
Scientific reports 2019 Mar 11;9(1):4032
Anti-high mobility group box-1 (HMGB1) antibody attenuates delayed cerebral vasospasm and brain injury after subarachnoid hemorrhage in rats.
Haruma J, Teshigawara K, Hishikawa T, Wang D, Liu K, Wake H, Mori S, Takahashi HK, Sugiu K, Date I, Nishibori M
Scientific reports 2016 Nov 24;6:37755
Scientific reports 2016 Nov 24;6:37755
Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH.
Moser B, Megerle A, Bekos C, Janik S, Szerafin T, Birner P, Schiefer AI, Mildner M, Lang I, Skoro-Sajer N, Sadushi-Kolici R, Taghavi S, Klepetko W, Ankersmit HJ
PloS one 2014;9(9):e106440
PloS one 2014;9(9):e106440
Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology.
Moser B, Janik S, Schiefer AI, Müllauer L, Bekos C, Scharrer A, Mildner M, Rényi-Vámos F, Klepetko W, Ankersmit HJ
PloS one 2014;9(4):e94118
PloS one 2014;9(4):e94118
IL-1α released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts.
Suwara MI, Green NJ, Borthwick LA, Mann J, Mayer-Barber KD, Barron L, Corris PA, Farrow SN, Wynn TA, Fisher AJ, Mann DA
Mucosal immunology 2014 May;7(3):684-93
Mucosal immunology 2014 May;7(3):684-93
Measles virus causes immunogenic cell death in human melanoma.
Donnelly OG, Errington-Mais F, Steele L, Hadac E, Jennings V, Scott K, Peach H, Phillips RM, Bond J, Pandha H, Harrington K, Vile R, Russell S, Selby P, Melcher AA
Gene therapy 2013 Jan;20(1):7-15
Gene therapy 2013 Jan;20(1):7-15
Human papillomavirus type 8 interferes with a novel C/EBPβ-mediated mechanism of keratinocyte CCL20 chemokine expression and Langerhans cell migration.
Sperling T, Ołdak M, Walch-Rückheim B, Wickenhauser C, Doorbar J, Pfister H, Malejczyk M, Majewski S, Keates AC, Smola S
PLoS pathogens 2012;8(7):e1002833
PLoS pathogens 2012;8(7):e1002833
Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis.
Liou TG, Adler FR, Keogh RH, Li Y, Jensen JL, Walsh W, Packer K, Clark T, Carveth H, Chen J, Rogers SL, Lane C, Moore J, Sturrock A, Paine R 3rd, Cox DR, Hoidal JR
PloS one 2012;7(8):e42748
PloS one 2012;7(8):e42748
Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients.
Colombo BM, Canevali P, Magnani O, Rossi E, Puppo F, Zocchi MR, Poggi A
PloS one 2012;7(2):e31903
PloS one 2012;7(2):e31903
Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease.
Vitali R, Stronati L, Negroni A, Di Nardo G, Pierdomenico M, del Giudice E, Rossi P, Cucchiara S
The American journal of gastroenterology 2011 Nov;106(11):2029-40
The American journal of gastroenterology 2011 Nov;106(11):2029-40
Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ
Journal of hepatology 2009 Aug;51(2):322-32
Journal of hepatology 2009 Aug;51(2):322-32
Potential role of high-mobility group box 1 in cystic fibrosis airway disease.
Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, O'Neal W, Sorscher EJ, Abraham E, Blalock JE
American journal of respiratory and critical care medicine 2008 Oct 15;178(8):822-31
American journal of respiratory and critical care medicine 2008 Oct 15;178(8):822-31
E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release.
Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP
International journal of cancer 2008 Oct 15;123(8):1741-50
International journal of cancer 2008 Oct 15;123(8):1741-50
Pivotal advance: inhibition of HMGB1 nuclear translocation as a mechanism for the anti-rheumatic effects of gold sodium thiomalate.
Zetterström CK, Jiang W, Wähämaa H, Ostberg T, Aveberger AC, Schierbeck H, Lotze MT, Andersson U, Pisetsky DS, Erlandsson Harris H
Journal of leukocyte biology 2008 Jan;83(1):31-8
Journal of leukocyte biology 2008 Jan;83(1):31-8
TLR4 enhances TGF-beta signaling and hepatic fibrosis.
Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF
Nature medicine 2007 Nov;13(11):1324-32
Nature medicine 2007 Nov;13(11):1324-32
IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response.
Rao DA, Tracey KJ, Pober JS
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6536-46
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human HMGB1/HMG-1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB1690) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HMGB1/HMG-1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- HMGB1/HMG-1 in CCD-18Co Human Cell Line. HMGB1/HMG-1 was detected in immersion fixed CCD-18Co human colonic fibroblast cell line using Mouse Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB1690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HMGB1 in HCT-116 Human Cell Line by Flow Cytometry. HCT-116 human colorectal carcinoma cell line was stained with Mouse Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB1690, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of HMGB1/HMG-1-regulated Genes by Chromatin Immunoprecipitation. HepG2 human hepatocellular carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HMGB1/HMG-1/DNA complexes were immunoprecipitated using 5 μg Mouse Anti-Human HMGB1/HMG-1 PE-conjugated Monoclonal Antibody (Catalog # MAB1690) or control antibody (Catalog # MAB004) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # BAF007). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The AFP promoter was detected by standard PCR.