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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- ARG65636 - Provider product page
- Provider
- Arigo
- Product name
- anti-HMGB1 antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide of human HMGB1
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1.8 mg/ml
- Storage
- Aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: 40 µg of lysates from: 1) Human hepatocellular carcinoma tissue, 2) HeLa cells, 3) Jurkat cells, 4) 293T cells, 5) Human breast infiltrative duct tissue stained with ARG65636 anti-HMGB1 antibody at 1/200 dilution. Exposure time: 1 minute.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of 1) Jurkat, 2) HeLa, 3) HUVEC, 4) Mouse spleen, 5) Mouse lung, 6) Mouse liver, 7) Rat spleen, and 8) Rat lung cell line lysates stained with ARG65636 anti-HMGB1 antibody at 1:200 dilution.Data provided by customer.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human thyroid cancer stained with ARG65636 anti-HMGB1 antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)
