- NB100-2322 - Novus Biologicals HMGB1 antibody

Submitted references The effect of high mobility group box-1 protein on cerebral edema, blood-brain barrier, oxidative stress and apoptosis in an experimental traumatic brain injury model.

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High-Mobility Group Box 1-Induced Complement Activation Causes Sterile Inflammation.

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The HMGB1/RAGE inflammatory pathway promotes pancreatic tumor growth by regulating mitochondrial bioenergetics.

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Adenovirus membrane penetration activates the NLRP3 inflammasome.

Barlan AU, Griffin TM, McGuire KA, Wiethoff CM
Journal of virology 2011 Jan;85(1):146-55

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: HMGB1/HMG-1 Antibody [NB100-2322] - Image shows a specific band for HMGB1 in 0.05 mg/mL of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Hepatocyte protein lysate at 1:1000 4C overnight. Image from verfified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Detection of HMGB1 in whole cell HeLa lysate using NB100-2322. ECL exposure: 1 min.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - Total protein from SHSY-5Y, MCF7, Neuro2A and HeLa was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-HMGB1 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: HMGB1/HMG-1 Antibody [NB100-2322] - The onset of innate immune response after I/R injury was compared in wt and PT-SSAT-Cko animals. Time course of the expression of HMGB1, TLR2 and 4 were compared in the kidneys of sham-operated and injured wt and PT-SSAT-Cko mice. The data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0110161) licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: ELISA: HMGB1/HMG-1 Antibody [NB100-2322] - A dose-dependent titration of the HRP conjugated anti-HMGB1 antibody on recombinant human HMGB1 protein. Image from verified customer review. Image using the HRP format of this antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NB100-2322] - Staining of HMGB1 in mouse liver using NB100-2322.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NB100-2322] - Rabbit blood vessel. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): HMGB1/HMG-1 Antibody [NB100-2322] - An intracellular stain was performed on HeLa with NB100-2322 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: HMGB1/HMG-1 Antibody [NB100-2322] - An intracellular stain was performed on RH-30 cells with NB100-2322F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.

NB100-2322

Antibody data