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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00003146-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003146-M02, RRID:AB_539583
- Product name
- Mouse Monoclonal HMGB1/HMG-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HMGB1 - high-mobility group box 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references HMGB1 secretion during cervical carcinogenesis promotes the acquisition of a tolerogenic functionality by plasmacytoid dendritic cells.
High expression of high-mobility group box 1 in the blood and lungs is associated with the development of chronic obstructive pulmonary disease in smokers.
TLR4, IL-6, IL-18, MyD88 and HMGB1 are highly expressed in intracranial inflammatory lesions and the IgG4/IgG ratio correlates with TLR4 and IL-6.
Human IgG antibody profiles differentiate between symptomatic patients with and without colorectal cancer.
Donor Toll-like receptor 4 contributes to ischemia and reperfusion injury following human kidney transplantation.
Demoulin S, Herfs M, Somja J, Roncarati P, Delvenne P, Hubert P
International journal of cancer 2015 Jul 15;137(2):345-58
International journal of cancer 2015 Jul 15;137(2):345-58
High expression of high-mobility group box 1 in the blood and lungs is associated with the development of chronic obstructive pulmonary disease in smokers.
Ko HK, Hsu WH, Hsieh CC, Lien TC, Lee TS, Kou YR
Respirology (Carlton, Vic.) 2014 Feb;19(2):253-261
Respirology (Carlton, Vic.) 2014 Feb;19(2):253-261
TLR4, IL-6, IL-18, MyD88 and HMGB1 are highly expressed in intracranial inflammatory lesions and the IgG4/IgG ratio correlates with TLR4 and IL-6.
Hirano H, Yoshioka T, Yunoue S, Fujio S, Yonezawa H, Niiro T, Habu M, Oyoshi T, Sugata S, Kamezawa T, Arimura H, Hanaya R, Tokimura H, Tokudome M, Arita K
Neuropathology : official journal of the Japanese Society of Neuropathology 2012 Dec;32(6):628-37
Neuropathology : official journal of the Japanese Society of Neuropathology 2012 Dec;32(6):628-37
Human IgG antibody profiles differentiate between symptomatic patients with and without colorectal cancer.
Kijanka G, Hector S, Kay EW, Murray F, Cummins R, Murphy D, MacCraith BD, Prehn JH, Kenny D
Gut 2010 Jan;59(1):69-78
Gut 2010 Jan;59(1):69-78
Donor Toll-like receptor 4 contributes to ischemia and reperfusion injury following human kidney transplantation.
Krüger B, Krick S, Dhillon N, Lerner SM, Ames S, Bromberg JS, Lin M, Walsh L, Vella J, Fischereder M, Krämer BK, Colvin RB, Heeger PS, Murphy BT, Schröppel B
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 3;106(9):3390-5
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 3;106(9):3390-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M02), clone 1D5.Lane 1: HMGB1 transfected lysate(24.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - HMGB1 monoclonal antibody (M02), clone 1D5 Analysis of HMGB1 expression in K-562.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of monoclonal antibody to HMGB1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. Antibody concentration 3 ug/ml.
