Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00003146-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003146-M04, RRID:AB_539585
- Product name
- Mouse Monoclonal HMGB1/HMG-1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. HMGB1 - high-mobility group box 1
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HMGB1/HMG-1 Antibody (1B2) [H00003146-M04] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M04), clone 1B2. Lane 1: HMGB1 transfected lysatE (24.9 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: HMGB1/HMG-1 Antibody (1B2) [H00003146-M04] - Detection limit for recombinant GST tagged HMGB1 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1B2) [H00003146-M04] - Analysis of monoclonal antibody to HMGB1 on HeLa cell. Antibody concentration 10 ug/ml