MAB1771

antibody from Abnova Corporation

Targeting: HMGB1 DKFZp686A04236, HMG1, HMG3, SBP-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1771

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1771, RRID:AB_1674713

Product name

HMGB1 monoclonal antibody, clone J2E1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against partial recombinant HMGB1.

Isotype

IgG

Antibody clone number

J2E1

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation.

Palumbo R, Sampaolesi M, De Marchis F, Tonlorenzi R, Colombetti S, Mondino A, Cossu G, Bianchi ME
The Journal of cell biology 2004 Feb 2;164(3):441-9

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HMGB1 as a DNA-binding cytokine.

Andersson U, Erlandsson-Harris H, Yang H, Tracey KJ
Journal of leukocyte biology 2002 Dec;72(6):1084-91

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Cell lysates of Jurkat (lane 1) and HeLa (lane 2) (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with HMGB1 monoclonal antibody, clone J2E1 (Cat # MAB1771) (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Paraffin embedded sections of human colon cancer tissue were incubated with HMGB1 monoclonal antibody, clone J2E1 (Cat # MAB1771) (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1 M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)