PA5-80691

antibody from Invitrogen Antibodies

Targeting: HMGB1 DKFZp686A04236, HMG1, HMG3, SBP-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80691

Provider

Invitrogen Antibodies

Product name

HMGB1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of HMGB1 in Lane A: HeLa Whole Cell Lysate (30 µg), Lane B: HepG2 Whole Cell Lysate (30 µg), Lane C: Jurkat Whole Cell Lysate (30 µg). Samples were probed using a HMGB1 Polyclonal Antibody (Product # PA5-80691) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:25 kDa. Observed band size:25 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using HMGB1 Polyclonal Antibody (Product # PA5-80691) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of HMGB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with HMGB1 Polyclonal Antibody (Product # PA5-80691, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HMGB1 in human liver with HMGB1 Polyclonal Antibody (Product # PA5-80691, 1:1,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HMGB1 in mouse kidney with HMGB1 Polyclonal Antibody (Product # PA5-80691, 1:1,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HMGB1 in rat kidney with HMGB1 Polyclonal Antibody (Product # PA5-80691, 1:1,000, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of HMGB1 in rat liver with HMGB1 Polyclonal Antibody (Product # PA5-80691, 1:1,000, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HMGB1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate, Lane B: 0.5 mg Jurkat Whole Cell Lysate, Lane C: 0.5 mg HepG2 Whole Cell Lysate 4 µL with HMGB1 Polyclonal Antibody (Product # PA5-80691) and 15 µL of 50 % Protein G agarose. Primary antibody: HMGB1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa.