66525-1-Ig

antibody from Proteintech Group

Targeting: HMGB1 DKFZp686A04236, HMG1, HMG3, SBP-1

Provider product page for 66525-1-Ig

Western blot

ELISA

Antibody data

Antibody Data
Antigen structure
References [59]
Comments [0]
Validations [0]

Submit

Product number: 66525-1-Ig - Provider product page
Provider: Proteintech Group
Product name: HMGB1 antibody
Antibody type: Monoclonal
Description: HMGB1 antibody (Cat. #66525-1-Ig) is a mouse monoclonal antibody that shows reactivity with Human, mouse and has been validated for the following applications: WB, ELISA.
Reactivity: Human, Mouse
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 1D12A6
Vial size: 20ul, 150ul

HMGB1 protein structure - 66525-1-Ig shown in red.

Submitted references Novel Dual-Pharmacophore TrxR Inhibitors Integrating Ferroptosis and Antitumor Immunity: A Closed-Loop Strategy for TIME Remodeling.

Xu Z, Yang Z, Lv L, Yang J, Liu W
Journal of medicinal chemistry 2026 May 14;69(9):10811-10827

Nitric Oxide Donor Alleviates Cardiac Arrest Induced Blood Brain Barrier Injury by Inhibiting HMGB1-ATG5 Mediated Endothelial Autophagy.

Duan P, Bi Y, Feng W, Jin Z, Meng Y, Li H, Li X, Luo L, Zhao X, Zhang B
Cellular and molecular neurobiology 2026 Mar 6;46(1)

Mild hypothermia alleviates ferroptosis in kidney ischemia-reperfusion injury via the glycolysis-lactate-HMGB1 lactylation axis.

Fu B, Fu Z, Liu Z, Deng Q, Cao F, Xiao J, Zhang X, Xiao Q
Cellular signalling 2026 Jul;143:112475

The Radioprotective Effects of (-)-Epigallocatechin-3-gallate (EGCG): Restoring the Gut Microbiota-Immune Axis to Ameliorate Radiation-Induced Intestinal Injury.

Gu J, Guo Q, Hua Q, Tang Z, Yang Y, Ji C, Yu J, Zhu D, Feng Q, Xu J, Zhao L
Dose-response : a publication of International Hormesis Society 2026 Jan-Mar;24(1):15593258261422635

Ultrasound-Targeted Nanobubbles Codelivering NKP-1339 and miR-142-5p for Synergistic Mitochondrial Immunogenic Cell Death and PD-L1 Inhibition in Cancer Therapy.

Zhang Y, Liu C, Jin S, Xie L, Xiao Q, Yao J
Biomaterials research 2025;29:0232

Lactate Facilitates Pancreatic Repair Following Acute Pancreatitis by Promoting Reparative Macrophage Polarization.

Jiang J, Wang R, Song P, Peng Q, Jin X, Li B, Ni J, Shen J, Bao J, Wu Z, Ge X, Wang X, Hu G
Cellular and molecular gastroenterology and hepatology 2025;19(9):101535

Curcumin Alleviates HMGB1-Mediated Inflammation Through the Signaling Pathway of TLR2-NF-κB in Bovine Ovarian Granulosa Cells.

Liu S, Xie Y, Wang L, Zhang J, Chen X, Feng X, Wang J, Zhang K, Li J
International journal of molecular sciences 2025 Sep 19;26(18)

NIR II-Guided Photoactivatable Silencing Polyplex Boosts Cancer Immunotherapy.

Zhang Y, Wang J, Zhang T, Tang D, Yang H, Guo S, Fan Y, Sun C, Xiao H, Huang Y, Weng Y
Exploration (Beijing, China) 2025 Oct;5(5):20240047

Lactylation of HMGB1 at K177 Drives Nuclear Export of TIAR to Promote Hypoxia-Induced Stress Granule Formation.

Li C, Liu Z, Zhu L, Wu G, Fu C, Li H, He T, Shen M, Liu H
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Nov;12(41):e04896

Resveratrol Promotes Wound Healing by Enhancing Angiogenesis via Inhibition of Ferroptosis.

Pan Y, Xia M, Luo J, Lu S
Food science & nutrition 2025 May;13(5):e70254

Single-cell analysis reveals that GFAP(+) dedifferentiated Schwann cells promote tumor progress in PNI-positive distal cholangiocarcinoma via lactate/HMGB1 axis.

Zu Z, Zhang C, Shi J, Chen K, Tang H, Hu K, Liu E, Ji C, Feng R, Shi X, Zhai W
Cell death & disease 2025 Mar 27;16(1):215

An antigen-capturing and lymph node-targeting nanoparticle for cancer immunotherapy.

Zhang Z, Xu C, Gong N, Qing G, Zhang Y, Shi Y, Brenner JS, Li F, Xu FJ, Liang XJ
Journal of controlled release : official journal of the Controlled Release Society 2025 Mar 10;379:993-1005

Circular RNA-OGDH promotes PANoptosis in diabetic cardiomyopathy: A novel mechanistic insight.

Guan J, Shi X, Ma J, Yin Y, Song G, Li Y, Chen X, Yan Y, Wang D, Liu S, Liu G, Zheng M, Ma F
The Journal of biological chemistry 2025 Jul;301(7):110280

Inhibition of ZFP281/ZNF281-RIPK1/RIPK3/MLKL signaling in hepatocytes by pterostilbene relieves hepatic lipometabolic disorder and inflammation in non-alcoholic steatohepatitis.

Lu C, Zhang Y, Miao J, Wei W, Wang Y, Han Y, Li Y, Tong Y, Wang T, Bao X
International immunopharmacology 2025 Jan 27;146:113936

Novel Indoleamine-2,3-Dioxygenase-Targeted Pt(IV) Prodrugs Regulate the Tumor Immune Microenvironment to Achieve Chemoimmunotherapy In Vitro and In Vivo.

Li Z, Liu XM, Tan F, Wang JQ, Qiao X, Feng YK, Xu JY, Hao JH
Journal of medicinal chemistry 2025 Feb 27;68(4):4352-4372

HMGB1 promotes LPS-induced M1 polarization and apoptosis in microglia by mediating the expression of immune and inflammation-related genes.

Zhao L, Huang Q, Li N, Lin W, Cui X, Yang F, Ma L, Zhang H, Ma H
Brain research bulletin 2025 Dec;233:111615

NIR-II photo-accelerated polymer nanoparticles boost tumor immunotherapy via PD-L1 silencing and immunogenic cell death.

Zhang T, Tang D, Wu P, Jiang S, Zhang Y, Naeem A, Li Y, Li C, Hu B, Guo S, Sun C, Xiao H, Yan R, Weng Y, Huang Y
Bioactive materials 2025 Apr;46:285-300

Nucleolin lactylation contributes to intrahepatic cholangiocarcinoma pathogenesis via RNA splicing regulation of MADD.

Yang L, Niu K, Wang J, Shen W, Jiang R, Liu L, Song W, Wang X, Zhang X, Zhang R, Wei D, Fan M, Jia L, Tao K
Journal of hepatology 2024 Oct;81(4):651-666

Glycyrrhizin Protects Submandibular Gland Against Radiation Damage by Enhancing Antioxidant Defense and Preserving Mitochondrial Homeostasis.

Zhou XR, Wang XY, Sun YM, Zhang C, Liu KJ, Zhang FY, Xiang B
Antioxidants & redox signaling 2024 Oct;41(10-12):723-743

Glycyrrhizin ameliorates colorectal cancer progression by regulating NHEJ pathway through inhibiting HMGB1-induced DNA damage response.

Han Y, Sheng W, Liu X, Liu H, Jia X, Li H, Wang C, Wang B, Hu T, Ma Y
Scientific reports 2024 Oct 23;14(1):24948

SIRT2 regulates high mobility group protein B1 nucleoplasmic shuttle and degradation via deacetylation in microglia.

Xing WQ, Piao XJ, Han Q, Shi HY, Wu WC, Si F, Lu JJ, Zhou TZ, Guo JR, Li SZ, Xu B
Journal of cellular physiology 2024 Nov;239(11):e31364

Enhancing cell pyroptosis with biomimetic nanoparticles for melanoma chemo-immunotherapy.

Sun S, He Y, Xu J, Leng S, Liu Y, Wan H, Yan L, Xu Y
Journal of controlled release : official journal of the Controlled Release Society 2024 Mar;367:470-485

Alpha-Emitter Radium-223 Induces STING-Dependent Pyroptosis to Trigger Robust Antitumor Immunity.

Yang M, Liu H, Lou J, Zhang J, Zuo C, Zhu M, Zhang X, Yin Y, Zhang Y, Qin S, Zhang H, Fan X, Dang Y, Cheng C, Cheng Z, Yu F
Small (Weinheim an der Bergstrasse, Germany) 2024 Mar;20(9):e2307448

Glioma-targeted oxaliplatin/ferritin clathrate reversing the immunosuppressive microenvironment through hijacking Fe(2+) and boosting Fenton reaction.

Li X, Cheng Y, Yang Z, Ji Q, Huan M, Ye W, Liu M, Zhang B, Liu D, Zhou S
Journal of nanobiotechnology 2024 Mar 5;22(1):93

Downregulation of HMGB1 carried by macrophage-derived extracellular vesicles delays atherosclerotic plaque formation through Caspase-11-dependent macrophage pyroptosis.

Liang W, Wei R, Zhu X, Li J, Lin A, Chen J, Wu W, Jie Q
Molecular medicine (Cambridge, Mass.) 2024 Mar 16;30(1):38

A manganese-oxide nano-rambutan as the intrinsic modifier for hypericin delivery and triple-negative breast cancer treatment.

Zhou W, Fan T, Yan Y, Chen Y, Ma X, Yang T, Xiang G, Lu Y
International journal of pharmaceutics 2024 Dec 5;666:124824

METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1.

Yang R, Yang C, Su D, Song Y, Min J, Qian Z, Shen X, Li J, Su H
Cancer gene therapy 2024 Apr;31(4):562-573

Expression of Multicopy Tandem Recombinant Ginseng Hexapeptide in Bacillus subtilis and the Evaluation of Antiaging Activity.

Xiang Q, Qu L, Lei H, Duan Z, Zhu C, Yuwen W, Ma X, Fan D
Journal of agricultural and food chemistry 2024 Apr 3;72(13):7266-7278

Structure and Function of RhoBTB1 Required for Substrate Specificity and Cullin-3 Ubiquitination.

Kumar G, Fang S, Golosova D, Lu KT, Brozoski DT, Vazirabad I, Sigmund CD
Function (Oxford, England) 2023;4(5):zqad034

Gankyrin-mediated interaction between cancer cells and tumor-associated macrophages facilitates prostate cancer progression and androgen deprivation therapy resistance.

Peng G, Wang C, Wang H, Qu M, Dong K, Yu Y, Jiang Y, Gan S, Gao X
Oncoimmunology 2023;12(1):2173422

PTMA binds to HMGB1 to regulate mitochondrial oxidative phosphorylation and thus affect the malignant progression of esophageal squamous cell carcinoma.

Chen S, He R, Lin X, Zhang W, Chen H, Xu R, Kang M
Journal of thoracic disease 2023 Mar 31;15(3):1302-1318

Neutrophil extracellular traps contribute to coagulopathy after traumatic brain injury.

Jin J, Wang F, Tian J, Zhao X, Dong J, Wang N, Liu Z, Zhao H, Li W, Mang G, Hu S
JCI insight 2023 Mar 22;8(6)

Chlorogenic Acid Alleviates Hepatic Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress, Inflammation, and Mitochondria-Mediated Apoptosis In Vivo and In Vitro.

Li K, Feng Z, Wang L, Ma X, Wang L, Liu K, Geng X, Peng C
Inflammation 2023 Jun;46(3):1061-1076

Erythrocyte membrane-camouflaged DNA-functionalized upconversion nanoparticles for tumor-targeted chemotherapy and immunotherapy.

Kou Q, Huang Y, Su Y, Lu L, Li X, Jiang H, Huang R, Li J, Nie X
Nanoscale 2023 Jun 1;15(21):9457-9476

Hypoxia-responsive Immunostimulatory Nanomedicines Synergize with Checkpoint Blockade Immunotherapy for Potentiating Cancer Immunotherapy.

Chen W, Sheng P, Chen Y, Liang Y, Wu S, Jia L, He X, Zhang CF, Wang CZ, Yuan CS
Chemical engineering journal (Lausanne, Switzerland : 1996) 2023 Jan;451(Pt 2)

Glycyrrhizin regulates the HMGB1/P38MAPK signalling pathway in status epilepticus.

Luo Z, Xu M, Zhang L, Zhang H, Xu Z, Xu Z
Molecular medicine reports 2023 Feb;27(2)

MIR-107/HMGB1/FGF-2 axis responds to excessive mechanical stretch to promote rapid repair of vascular endothelial cells.

Ma H, Wang L, Sun H, Yu Q, Yang T, Wang Y, Niu B, Jia Y, Liu Y, Liang Z, An M, Guo J
Archives of biochemistry and biophysics 2023 Aug;744:109686

A marine-derived small molecule induces immunogenic cell death against triple-negative breast cancer through ER stress-CHOP pathway.

Wen H, Zhong Y, Yin Y, Qin K, Yang L, Li D, Yu W, Yang C, Deng Z, Hong K
International journal of biological sciences 2022;18(7):2898-2913

ORFV infection enhances CXCL16 secretion and causes oncolysis of lung cancer cells through immunogenic apoptosis.

Wang R, Mo J, Luo X, Zhang G, Liu F, Luo S
Frontiers in cellular and infection microbiology 2022;12:910466

A protein-based cGAS-STING nanoagonist enhances T cell-mediated anti-tumor immune responses.

Wang X, Liu Y, Xue C, Hu Y, Zhao Y, Cai K, Li M, Luo Z
Nature communications 2022 Sep 28;13(1):5685

Laminar shear stress inhibits inflammation by activating autophagy in human aortic endothelial cells through HMGB1 nuclear translocation.

Meng Q, Pu L, Qi M, Li S, Sun B, Wang Y, Liu B, Li F
Communications biology 2022 May 6;5(1):425

Magnetic hyperthermia induces effective and genuine immunogenic tumor cell death with respect to exogenous heating.

Yan B, Liu C, Wang S, Li H, Jiao J, Lee WSV, Zhang S, Hou Y, Hou Y, Ma X, Fan H, Lv Y, Liu X
Journal of materials chemistry. B 2022 Jul 20;10(28):5364-5374

NMN recruits GSH to enhance GPX4-mediated ferroptosis defense in UV irradiation induced skin injury.

Feng Z, Qin Y, Huo F, Jian Z, Li X, Geng J, Li Y, Wu J
Biochimica et biophysica acta. Molecular basis of disease 2022 Jan 1;1868(1):166287

Preventive effect of sanguinarine on intestinal injury in mice exposed to whole abdominal irradiation.

Gu J, Zhao L, Chen YZ, Guo YX, Sun Y, Guo Q, Duan GX, Li C, Tang ZB, Zhang ZX, Qin LQ, Xu JY
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2022 Feb;146:112496

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Xu Z, Xu J, Sun S, Lin W, Li Y, Lu Q, Li F, Yang Z, Lu Y, Liu W
Redox biology 2022 Aug;54:102351

Paraquat Inhibits Autophagy Via Intensifying the Interaction Between HMGB1 and α-Synuclein.

Wang K, Zhang B, Zhang B, Wu K, Tian T, Yan W, Huang M
Neurotoxicity research 2022 Apr;40(2):520-529

Anti-PD-L1 DNAzyme Loaded Photothermal Mn(2+) /Fe(3+) Hybrid Metal-Phenolic Networks for Cyclically Amplified Tumor Ferroptosis-Immunotherapy.

Liu P, Shi X, Peng Y, Hu J, Ding J, Zhou W
Advanced healthcare materials 2022 Apr;11(8):e2102315

The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.

Du Y, Zhong F, Cheng H, Li T, Chen Y, Tan P, Huang M, Liang T, Liu Y, Xia X, Fu W
Oxidative medicine and cellular longevity 2021;2021:4628050

High Mobility Group Box 1 Promotes Lung Cancer Cell Migration and Motility via Regulation of Dynamin-Related Protein 1.

Liu WL, Li CY, Cheng WC, Chang CY, Chen YH, Lu CY, Wang SC, Liu YR, Cheng MH, Chong IW, Liu PL
International journal of molecular sciences 2021 Mar 31;22(7)

Anti-Oxidative and Immuno-Protective Effect of Camel Milk on Radiation-Induced Intestinal Injury in C57BL/6 J Mice.

Chen YZ, Li C, Gu J, Lv SC, Song JY, Tang ZB, Duan GX, Qin LQ, Zhao L, Xu JY
Dose-response : a publication of International Hormesis Society 2021 Jan-Mar;19(1):15593258211003798

Cascade-Responsive Hierarchical Nanosystems for Multisite Specific Drug Exposure and Boosted Chemoimmunotherapy.

Zhang J, Zhang Y, Zhao B, Lv M, Chen E, Zhao C, Jiang L, Qian H, Huang D, Zhong Y, Chen W
ACS applied materials & interfaces 2021 Dec 15;13(49):58319-58328

Bone marrow mesenchymal stem cell exosomes suppress phosphate-induced aortic calcification via SIRT6-HMGB1 deacetylation.

Wei W, Guo X, Gu L, Jia J, Yang M, Yuan W, Rong S
Stem cell research & therapy 2021 Apr 13;12(1):235

The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model.

Cui H, Cheng Y, He Y, Cheng W, Zhao W, Zhao H, Zhou FH, Wang L, Dong J, Cai S
Molecular medicine reports 2020 Nov;22(5):3723-3734

Saikosaponin-d attenuated lipopolysaccharide-induced depressive-like behaviors via inhibiting microglia activation and neuroinflammation.

Su J, Pan YW, Wang SQ, Li XZ, Huang F, Ma SP
International immunopharmacology 2020 Mar;80:106181

HMGB1 Mediates Paraquat-Induced Neuroinflammatory Responses via Activating RAGE Signaling Pathway.

Huang M, Guo M, Wang K, Wu K, Li Y, Tian T, Wang Y, Yan W, Zhou Z, Yang H
Neurotoxicity research 2020 Apr;37(4):913-925

Lysophosphatidylcholine Induces NLRP3 Inflammasome-Mediated Foam Cell Formation and Pyroptosis in Human Monocytes and Endothelial Cells.

Corrêa R, Silva LFF, Ribeiro DJS, Almeida RDN, Santos IO, Corrêa LH, de Sant'Ana LP, Assunção LS, Bozza PT, Magalhães KG
Frontiers in immunology 2019;10:2927

SIRT1-regulated HMGB1 release is partially involved in TLR4 signal transduction: A possible anti-neuroinflammatory mechanism of resveratrol in neonatal hypoxic-ischemic brain injury.

Le K, Chibaatar Daliv E, Wu S, Qian F, Ali AI, Yu D, Guo Y
International immunopharmacology 2019 Oct;75:105779

Chloroquine ameliorates carbon tetrachloride-induced acute liver injury in mice via the concomitant inhibition of inflammation and induction of apoptosis.

Dai C, Xiao X, Li D, Tun S, Wang Y, Velkov T, Tang S
Cell death & disease 2018 Nov 26;9(12):1164

Treatment of colon cancer with liver X receptor agonists induces immunogenic cell death.

Wang Q, Ren M, Feng F, Chen K, Ju X
Molecular carcinogenesis 2018 Jul;57(7):903-910

No comments: Submit comment

No validations: Submit validation data