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10829-1-AP

antibody from Invitrogen Antibodies

Targeting: HMGB1 DKFZp686A04236, HMG1, HMG3, SBP-1

Provider product page for 10829-1-AP

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Chromatin Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [0]
Comments [0]
Validations
Western blot [9]
Immunocytochemistry [1]
Immunohistochemistry [2]
Other assay [1]

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Validation data

Product number: 10829-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: HMGB1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.24 mg/mL
Storage: -20°C

HMGB1 protein structure - 10829-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human testis tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: WB result of HMGB1 antibody (10829-1-AP, 1:1500) with si-control and si-HMGB1 transfected Jurkat cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human brain tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat brain tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat stomach tissue were subjected to SDS PAGE followed by western blot with 10829-1-AP (HMGB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.