Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [11]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-16263 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGB1 Monoclonal Antibody (19N10B7)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 19N10B7
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in full-length human HMGB1 protein, human Jurkat cell lysate and mouse NIH 3T3 cell lysate. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 2 µg/mL followed by a Goat anti-mouse Ig HRP secondary antibody. Lane A: Full-length human HMGB1 protein; Lane B: Human Jurkat cell lysate; Lane C: Mouse NIH 3T3 cell lysate. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in 1.0 mg/mL HeLa lysate. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed paraffin-embedded tissue section of normal human skin. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL. The various cells of the epidermal layer showed intense nuclear staining along with weak cytoplasmic staining. The blood vessels, glandular cells and the other cells in dermal layer also showed nuclear positivity for HMGB1 immunostaining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed paraffin-embedded tissue section of human stomach. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL. Distinct nuclear staining of HMGB1 was observed in different cells of the glandular stomach.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed, paraffin-embedded human breast. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed paraffin-embedded tissue section of human bladder cancer. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL. Strong nuclear HMGB1 immunopositivity was observed in the bladder cancer cells whereas the staining was weak in cells of tumor stroma.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed, paraffin-embedded human spleen tissue. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL followed by peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed, paraffin-embedded human liver tissue. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL followed by peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed paraffin-embedded tissue section of malignant stromal tumor of small bowel from human. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL. HMGB1 immunopositivity of differential intensity was observed in the cells of tested section.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed paraffin-embedded tissue section of human stomach cancer. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL. This representative image shows a distinct nuclear HMGB1 immunopositivity in the cancerous and sub-mucosal cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed paraffin-embedded human prostate tissue. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 10 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed paraffin-embedded tissue section of normal human colon. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL. Representative image shows specific HMGB1 nuclear positivity in different mucosal cells of the colon.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of HMGB1 in formalin-fixed paraffin-embedded tissue section of normal human brain. Samples were incubated in HMGB1 monoclonal antibody (Product # MA5-16263) using a dilution of 5 µg/mL. Representative image shows a distinct nuclear immunostaining of HMGB1 in the various brain cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular flow cytometric analysis using HMGB1 monoclonal antibody (Product # MA5-16263). Human Jurkat cells were probed using 0.5 µg of HMGB1 monoclonal antibody (Product # MA5-16263) (red) and 0.5 µg of isotype control antibody (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of HMGB1 in Human Jurkat cells. Samples were incubated in HMGB1 monoclonal (Product # MA5-16263) using a dilution of 0.5 µg. Antibody (red) and 0.5 µg of isotype control antibody (green).
