Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-23487 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in Human ThP1 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse NIH 3T3 cell lysate were using a HMGB1 polyclonal antibody (Product # PA5-23487) at 5 µg/mL. Goat anti-rabbit Ig HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate. Samples were incubated in HMGB1 polyclonal antibody (Product # PA5-23487) using a dilution of 5 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGB1 in 0.5 mg/mL Jurkat lysate. Samples were incubated in HMGB1 polyclonal antibody (Product # PA5-23487). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular flow cytometric analysis using HMGB1 polyclonal antibody (Product # PA5-23487). Human monocytes were probed using 0.5 µg of HMGB1 polyclonal antibody (Product # PA5-23487) (red) and 0.5 µg of isotype control antibody (green).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of HMGB1 in Human monocytes. Samples were incubated in HMGB1 polyclonal antibody (Product # PA5-23487) using a dilution of 0.5 µg followed by APC secondary. Antibody (red) and 0.5 µg of isotype control antibody (green).