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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- GTX628835 - Provider product page
- Provider
- GeneTex
- Product name
- HMGB1 antibody [GT348]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
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- Enhanced method
- Genetic validation
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- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT348] (GTX628835) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT348] detects HMGB1 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extract C. 30 ?g HepG2 whole cell lysate/extract12% SDS-PAGEHMGB1 antibody [GT348] (GTX628835) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT348] detects HMGB1 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extractB. 30 ?g C8D30 whole cell lysate/extractC. 30 ?g NIH-3T3 whole cell lysate/extractD. 30 ?g BCL-1 whole cell lysate/extractE. 30 ?g Raw264.7 whole cell lysate/extractF. 30 ?g C2C12 whole cell lysate/extract12% SDS-PAGEHMGB1 antibody [GT348] (GTX628835) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT348] detects HMGB1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat-2 whole cell lysate/extract12% SDS-PAGEHMGB1 antibody [GT348] (GTX628835) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT348] (GTX628835) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with HMGB1 antibody [GT348] (GTX628835) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT348] detects HMGB1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGB1 protein stained by HMGB1 antibody [GT348] (GTX628835) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Supportive validation
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- Main image
- Experimental details
- HMGB1 antibody [GT348] detects HMGB1 protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HMGB1 antibody [GT348] (GTX628835) dilution: 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT348] detects HMGB1 protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. HMGB1 antibody [GT348] (GTX628835) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT348] detects HMGB1 protein at nucleus on mouse lymph node by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. HMGB1 antibody [GT348] (GTX628835) dilution: 1:500.
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- GeneTex (provider)
- Main image
- Experimental details
- HMGB1 antibody [GT348] detects HMGB1 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: HMGB1 protein stained by HMGB1 antibody [GT348] (GTX628835) diluted at 1:250.Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
