PA1-1755

antibody from Invitrogen Antibodies

Targeting: ITIH1 H1P, IATIH, ITIH

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-1755

Provider

Invitrogen Antibodies

Product name

ITIH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

PA1-1755 detects HC1 from human and mouse samples.

Concentration

1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ITIH1 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ITIH1 Rabbit Polyclonal Antibody (Product # PA1-1755) at 2µg/mLin 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ITIH1 using a polyclonal antibody (Product # PA1-1755).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 illustrates immunostaining of HC1 on paraffin embedded human OA chondrocyte tissue using Product # PA1-1755. HC1 is stained in red, hyaluronan in green, and the nuclei in blue.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 illustrates immunostaining of HC1 on paraffin embedded mouse OA periosteum tissue using Product # PA1-1755. HC1 is stained in red, hyaluronan in green, and the nuclei in blue.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunostaining of HC1 on paraffin embedded human OA chondrocyte tissue using Product # PA1-1755. HC1 is stained in red, hyaluronan in green, and the nuclei in blue.