Antibody data
- Antibody Data
- Antigen structure
- References [37]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- 14711-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14711-1-AP, RRID:AB_2143063
- Product name
- MGEA5 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated MGEA5 antibody (Cat. #14711-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references DOT1L O-GlcNAcylation promotes its protein stability and MLL-fusion leukemia cell proliferation.
Repeated hypoxia exposure induces cognitive dysfunction, brain inflammation, and amyloidβ/p-Tau accumulation through reduced brain O-GlcNAcylation in zebrafish.
O‑GlcNAcylation contributes to intermittent hypoxia‑associated vascular dysfunction via modulation of MAPKs but not CaMKII pathways.
O-GlcNAcylation of TDP-43 suppresses proteinopathies and promotes TDP-43's mRNA splicing activity.
Quantitative chemoproteomics reveals O-GlcNAcylation of cystathionine γ-lyase (CSE) represses trophoblast syncytialization.
OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.
Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.
REM Sleep Deprivation Impairs Learning and Memory by Decreasing Brain O-GlcNAc Cycling in Mouse.
Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.
Sleep deprivation impairs learning and memory by decreasing protein O-GlcNAcylation in the brain of adult zebrafish.
Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.
Elevated O-GlcNAcylation induces an antidepressant-like phenotype and decreased inhibitory transmission in medial prefrontal cortex.
Reduced O-GlcNAcylation and tubular hypoxia contribute to the antifibrotic effect of SGLT2 inhibitor dapagliflozin in the diabetic kidney.
Modulation of O-GlcNAcylation Regulates Autophagy in Cortical Astrocytes.
O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion.
Glucosamine improves survival in a mouse model of sepsis and attenuates sepsis-induced lung injury and inflammation.
O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signaling.
High O-linked N-acetylglucosamine transferase expression predicts poor survival in patients with early stage lung adenocarcinoma.
Identification and characterization of a missense mutation in the O-linked β-N-acetylglucosamine (O-GlcNAc) transferase gene that segregates with X-linked intellectual disability.
Mutations in N-acetylglucosamine (O-GlcNAc) transferase in patients with X-linked intellectual disability.
Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.
Differential membranous E-cadherin expression, cell proliferation and O-GlcNAcylation between primary and metastatic nodal lesion in colorectal cancer.
Role of O-linked N-acetylglucosamine modification in diabetic nephropathy.
Removal of O-GlcNAcylation is important for pig preimplantation development.
mTOR/MYC Axis Regulates O-GlcNAc Transferase Expression and O-GlcNAcylation in Breast Cancer.
In vivo and in vitro evidence that chronic activation of the hexosamine biosynthetic pathway interferes with leptin-dependent STAT3 phosphorylation.
Conditional knock-out reveals a requirement for O-linked N-Acetylglucosaminase (O-GlcNAcase) in metabolic homeostasis.
Alterations in left ventricular function during intermittent hypoxia: Possible involvement of O-GlcNAc protein and MAPK signaling.
Hexosamine biosynthetic pathway activity in leptin resistant sucrose-drinking rats.
O-GlcNAc modification of the runt-related transcription factor 2 (Runx2) links osteogenesis and nutrient metabolism in bone marrow mesenchymal stem cells.
O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development.
Hyper-O-GlcNAcylation is anti-apoptotic and maintains constitutive NF-κB activity in pancreatic cancer cells.
Epigenetic switching by the metabolism-sensing factors in the generation of orexin neurons from mouse embryonic stem cells.
Identification of O-linked N-acetylglucosamine (O-GlcNAc)-modified osteoblast proteins by electron transfer dissociation tandem mass spectrometry reveals proteins critical for bone formation.
O-GlcNAcylation plays a role in tumor recurrence of hepatocellular carcinoma following liver transplantation.
Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells.
A lipid-droplet-targeted O-GlcNAcase isoform is a key regulator of the proteasome.
Song T, Zou Q, Yan Y, Lv S, Li N, Zhao X, Ma X, Liu H, Tang B, Sun L
Cell reports 2021 Sep 21;36(12):109739
Cell reports 2021 Sep 21;36(12):109739
Repeated hypoxia exposure induces cognitive dysfunction, brain inflammation, and amyloidβ/p-Tau accumulation through reduced brain O-GlcNAcylation in zebrafish.
Park J, Jung S, Kim SM, Park IY, Bui NA, Hwang GS, Han IO
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2021 Nov;41(11):3111-3126
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2021 Nov;41(11):3111-3126
O‑GlcNAcylation contributes to intermittent hypoxia‑associated vascular dysfunction via modulation of MAPKs but not CaMKII pathways.
Guo X, Deng Y, Zhan L, Shang J, Liu H
Molecular medicine reports 2021 Nov;24(5)
Molecular medicine reports 2021 Nov;24(5)
O-GlcNAcylation of TDP-43 suppresses proteinopathies and promotes TDP-43's mRNA splicing activity.
Zhao MJ, Yao X, Wei P, Zhao C, Cheng M, Zhang D, Xue W, He WT, Xue W, Zuo X, Jiang LL, Luo Z, Song J, Shu WJ, Yuan HY, Liang Y, Sun H, Zhou Y, Zhou Y, Zheng L, Hu HY, Wang J, Du HN
EMBO reports 2021 Jun 4;22(6):e51649
EMBO reports 2021 Jun 4;22(6):e51649
Quantitative chemoproteomics reveals O-GlcNAcylation of cystathionine γ-lyase (CSE) represses trophoblast syncytialization.
Liu J, Shao X, Qin W, Zhang Y, Dang F, Yang Q, Yu X, Li YX, Chen X, Wang C, Wang YL
Cell chemical biology 2021 Jun 17;28(6):788-801.e5
Cell chemical biology 2021 Jun 17;28(6):788-801.e5
OGA is associated with deglycosylation of NONO and the KU complex during DNA damage repair.
Cui Y, Xie R, Zhang X, Liu Y, Hu Y, Li Y, Liu X, Yu X, Wu C
Cell death & disease 2021 Jun 16;12(7):622
Cell death & disease 2021 Jun 16;12(7):622
Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.
Ou W, Liang Y, Qin Y, Wu W, Xie M, Zhang Y, Zhang Y, Ji L, Yu H, Li T
Redox biology 2021 Jul;43:101994
Redox biology 2021 Jul;43:101994
REM Sleep Deprivation Impairs Learning and Memory by Decreasing Brain O-GlcNAc Cycling in Mouse.
Kim SM, Zhang S, Park J, Sung HJ, Tran TT, Chung C, Han IO
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2021 Jul 26;
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2021 Jul 26;
Traditional herbal formula Wu-Mei-Wan alleviates TNBS-induced colitis in mice by inhibiting necroptosis through increasing RIPK3 O-GlcNAcylation.
Wu F, Shao Q, Cheng Z, Xiong X, Fang K, Zhao Y, Dong R, Xu L, Lu F, Chen G
Chinese medicine 2021 Aug 16;16(1):78
Chinese medicine 2021 Aug 16;16(1):78
Sleep deprivation impairs learning and memory by decreasing protein O-GlcNAcylation in the brain of adult zebrafish.
Lee Y, Park J, Kim SM, Nam K, Lee W, Cho H, Han IO
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):853-864
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jan;34(1):853-864
Disruption of retinoid homeostasis induces RBP4 overproduction in diabetes: O-GlcNAcylation involved.
Shin SJ, Chen CH, Kuo WC, Chan HC, Chan HC, Lin KD, Ke LY
Metabolism: clinical and experimental 2020 Dec;113:154403
Metabolism: clinical and experimental 2020 Dec;113:154403
Elevated O-GlcNAcylation induces an antidepressant-like phenotype and decreased inhibitory transmission in medial prefrontal cortex.
Cho Y, Hwang H, Rahman MA, Chung C, Rhim H
Scientific reports 2020 Apr 24;10(1):6924
Scientific reports 2020 Apr 24;10(1):6924
Reduced O-GlcNAcylation and tubular hypoxia contribute to the antifibrotic effect of SGLT2 inhibitor dapagliflozin in the diabetic kidney.
Hodrea J, Balogh DB, Hosszu A, Lenart L, Besztercei B, Koszegi S, Sparding N, Genovese F, Wagner LJ, Szabo AJ, Fekete A
American journal of physiology. Renal physiology 2020 Apr 1;318(4):F1017-F1029
American journal of physiology. Renal physiology 2020 Apr 1;318(4):F1017-F1029
Modulation of O-GlcNAcylation Regulates Autophagy in Cortical Astrocytes.
Rahman MA, Hwang H, Cho Y, Rhim H
Oxidative medicine and cellular longevity 2019;2019:6279313
Oxidative medicine and cellular longevity 2019;2019:6279313
O‑GlcNAc modification influences endometrial receptivity by promoting endometrial cell proliferation, migration and invasion.
Han X, Li X, Liu H, Zhang H, Li A, Dong M, Xu Y, Yan B, Sui L, Kong Y
Oncology reports 2019 Nov;42(5):2065-2074
Oncology reports 2019 Nov;42(5):2065-2074
Glucosamine improves survival in a mouse model of sepsis and attenuates sepsis-induced lung injury and inflammation.
Hwang JS, Kim KH, Park J, Kim SM, Cho H, Lee Y, Han IO
The Journal of biological chemistry 2019 Jan 11;294(2):608-622
The Journal of biological chemistry 2019 Jan 11;294(2):608-622
O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signaling.
Selvan N, George S, Serajee FJ, Shaw M, Hobson L, Kalscheuer V, Prasad N, Levy SE, Taylor J, Aftimos S, Schwartz CE, Huq AM, Gecz J, Wells L
The Journal of biological chemistry 2018 Jul 6;293(27):10810-10824
The Journal of biological chemistry 2018 Jul 6;293(27):10810-10824
High O-linked N-acetylglucosamine transferase expression predicts poor survival in patients with early stage lung adenocarcinoma.
Lin YC, Lin CH, Yeh YC, Ho HL, Wu YC, Chen MY, Chou TY
Oncotarget 2018 Jul 24;9(57):31032-31044
Oncotarget 2018 Jul 24;9(57):31032-31044
Identification and characterization of a missense mutation in the O-linked β-N-acetylglucosamine (O-GlcNAc) transferase gene that segregates with X-linked intellectual disability.
Vaidyanathan K, Niranjan T, Selvan N, Teo CF, May M, Patel S, Weatherly B, Skinner C, Opitz J, Carey J, Viskochil D, Gecz J, Shaw M, Peng Y, Alexov E, Wang T, Schwartz C, Wells L
The Journal of biological chemistry 2017 May 26;292(21):8948-8963
The Journal of biological chemistry 2017 May 26;292(21):8948-8963
Mutations in N-acetylglucosamine (O-GlcNAc) transferase in patients with X-linked intellectual disability.
Willems AP, Gundogdu M, Kempers MJE, Giltay JC, Pfundt R, Elferink M, Loza BF, Fuijkschot J, Ferenbach AT, van Gassen KLI, van Aalten DMF, Lefeber DJ
The Journal of biological chemistry 2017 Jul 28;292(30):12621-12631
The Journal of biological chemistry 2017 Jul 28;292(30):12621-12631
Aberrant Glycosylation in the Left Ventricle and Plasma of Rats with Cardiac Hypertrophy and Heart Failure.
Nagai-Okatani C, Minamino N
PloS one 2016;11(6):e0150210
PloS one 2016;11(6):e0150210
Differential membranous E-cadherin expression, cell proliferation and O-GlcNAcylation between primary and metastatic nodal lesion in colorectal cancer.
Jang TJ
Pathology, research and practice 2016 Feb;212(2):113-9
Pathology, research and practice 2016 Feb;212(2):113-9
Role of O-linked N-acetylglucosamine modification in diabetic nephropathy.
Gellai R, Hodrea J, Lenart L, Hosszu A, Koszegi S, Balogh D, Ver A, Banki NF, Fulop N, Molnar A, Wagner L, Vannay A, Szabo AJ, Fekete A
American journal of physiology. Renal physiology 2016 Dec 1;311(6):F1172-F1181
American journal of physiology. Renal physiology 2016 Dec 1;311(6):F1172-F1181
Removal of O-GlcNAcylation is important for pig preimplantation development.
Shibutani M, Mori T, Miyano T, Miyake M
The Journal of reproduction and development 2015;61(4):341-50
The Journal of reproduction and development 2015;61(4):341-50
mTOR/MYC Axis Regulates O-GlcNAc Transferase Expression and O-GlcNAcylation in Breast Cancer.
Sodi VL, Khaku S, Krutilina R, Schwab LP, Vocadlo DJ, Seagroves TN, Reginato MJ
Molecular cancer research : MCR 2015 May;13(5):923-33
Molecular cancer research : MCR 2015 May;13(5):923-33
In vivo and in vitro evidence that chronic activation of the hexosamine biosynthetic pathway interferes with leptin-dependent STAT3 phosphorylation.
Zimmerman AD, Harris RB
American journal of physiology. Regulatory, integrative and comparative physiology 2015 Mar 15;308(6):R543-55
American journal of physiology. Regulatory, integrative and comparative physiology 2015 Mar 15;308(6):R543-55
Conditional knock-out reveals a requirement for O-linked N-Acetylglucosaminase (O-GlcNAcase) in metabolic homeostasis.
Keembiyehetty C, Love DC, Harwood KR, Gavrilova O, Comly ME, Hanover JA
The Journal of biological chemistry 2015 Mar 13;290(11):7097-113
The Journal of biological chemistry 2015 Mar 13;290(11):7097-113
Alterations in left ventricular function during intermittent hypoxia: Possible involvement of O-GlcNAc protein and MAPK signaling.
Guo X, Shang J, Deng Y, Yuan X, Zhu D, Liu H
International journal of molecular medicine 2015 Jul;36(1):150-8
International journal of molecular medicine 2015 Jul;36(1):150-8
Hexosamine biosynthetic pathway activity in leptin resistant sucrose-drinking rats.
Harris RB, Apolzan JW
Physiology & behavior 2015 Jan;138:208-18
Physiology & behavior 2015 Jan;138:208-18
O-GlcNAc modification of the runt-related transcription factor 2 (Runx2) links osteogenesis and nutrient metabolism in bone marrow mesenchymal stem cells.
Nagel AK, Ball LE
Molecular & cellular proteomics : MCP 2014 Dec;13(12):3381-95
Molecular & cellular proteomics : MCP 2014 Dec;13(12):3381-95
O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development.
Radermacher PT, Myachina F, Bosshardt F, Pandey R, Mariappa D, Müller HA, Lehner CF
Proceedings of the National Academy of Sciences of the United States of America 2014 Apr 15;111(15):5592-7
Proceedings of the National Academy of Sciences of the United States of America 2014 Apr 15;111(15):5592-7
Hyper-O-GlcNAcylation is anti-apoptotic and maintains constitutive NF-κB activity in pancreatic cancer cells.
Ma Z, Vocadlo DJ, Vosseller K
The Journal of biological chemistry 2013 May 24;288(21):15121-30
The Journal of biological chemistry 2013 May 24;288(21):15121-30
Epigenetic switching by the metabolism-sensing factors in the generation of orexin neurons from mouse embryonic stem cells.
Hayakawa K, Hirosawa M, Tabei Y, Arai D, Tanaka S, Murakami N, Yagi S, Shiota K
The Journal of biological chemistry 2013 Jun 14;288(24):17099-110
The Journal of biological chemistry 2013 Jun 14;288(24):17099-110
Identification of O-linked N-acetylglucosamine (O-GlcNAc)-modified osteoblast proteins by electron transfer dissociation tandem mass spectrometry reveals proteins critical for bone formation.
Nagel AK, Schilling M, Comte-Walters S, Berkaw MN, Ball LE
Molecular & cellular proteomics : MCP 2013 Apr;12(4):945-55
Molecular & cellular proteomics : MCP 2013 Apr;12(4):945-55
O-GlcNAcylation plays a role in tumor recurrence of hepatocellular carcinoma following liver transplantation.
Zhu Q, Zhou L, Yang Z, Lai M, Xie H, Wu L, Xing C, Zhang F, Zheng S
Medical oncology (Northwood, London, England) 2012 Jun;29(2):985-93
Medical oncology (Northwood, London, England) 2012 Jun;29(2):985-93
Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells.
Myers SA, Panning B, Burlingame AL
Proceedings of the National Academy of Sciences of the United States of America 2011 Jun 7;108(23):9490-5
Proceedings of the National Academy of Sciences of the United States of America 2011 Jun 7;108(23):9490-5
A lipid-droplet-targeted O-GlcNAcase isoform is a key regulator of the proteasome.
Keembiyehetty CN, Krzeslak A, Love DC, Hanover JA
Journal of cell science 2011 Aug 15;124(Pt 16):2851-60
Journal of cell science 2011 Aug 15;124(Pt 16):2851-60
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human brain tissue were subjected to SDS PAGE followed by western blot with 14711-1-AP(MGEA5 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using MGEA5 antibody 14711-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-MGEA5 (IP:14711-1-AP, 3ug; Detection:14711-1-AP 1:500) with SH-SY5Y cells lysate 2900ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human endometrial cancer using 14711-1-AP(MGEA5 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human endometrial cancer using 14711-1-AP(MGEA5 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue