Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-67426 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mgea5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DCISDIAPMQT DEQTNKEQFV PGPNEKPLYT AEPVTLEDLQ LLADLFYLPY EHGPKGAQML REFQWLRANS SVVSVNCKGK D
- Concentration
- 0.4 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Mgea5 was achieved by transfecting HeLa cells with Mgea5 specific siRNAs (Silencer® select Product # s21067, s21066). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the Mgea5 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Mgea5 Polyclonal Antibody (Product # PA5-67426, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Mgea5.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Mgea5 Polyclonal Antibody (Product # PA5-67426) and a 120 kDa band corresponding to Mgea5 was observed across cell lines tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), A549 (Lane 3), A-431 (Lane 4), SH-SY5Y (Lane 5), PC-12 (Lane 6), Mouse Brain (Lane 7) and Rat Brain (Lane 8) were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Mgea5 in human cell line U-2 OS shows localization to cytosol. Samples were probed using a Mgea5 Polyclonal Antibody (Product # PA5-67426).