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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [4]
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- Product number
- PA5-57540 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Mgea5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: WTGPKVVSKE IPVESIEEVS KIIKRAPVIW DNIHANDYDQ KRLFLGPYKG RSTELIPRLK GVLTNPNCEF EANYVAIHTL ATWYKSNMNG VRKDVVM
- Concentration
- 0.09 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Mgea5 in human cell line A-431 using a Mgea5 Polyclonal Antibody (Product # PA5-57540).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Mgea5 in mouse cell line NIH-3T3 and rat cell line NBT-II using a Mgea5 Polyclonal Antibody (Product # PA5-57540).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Mgea5 was achieved by transfecting HeLa cells with Mgea5 specific siRNAs (Silencer® select Product # s21067, s21066). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the Mgea5 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Mgea5 Polyclonal Antibody (Product # PA5-57540, 0.5ug/ml) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Mgea5.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Mgea5 Polyclonal Antibody (Product # PA5-57540) and a 120 kDa band corresponding to Mgea5 was observed across cell lines and tissues tested. Modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), U-2 OS (Lane 2), A549 (Lane 3), A-431 (Lane 4), SH-SY5Y (Lane 5), PC-12 (Lane 6), Mouse Brain (Lane 7) and Rat Brain (Lane 8) were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 ug/ml) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Mgea5 in human testis using Mgea5 Polyclonal Antibody (Product # PA5-57540) shows moderate to strong cytoplasmic positivity in spermatogonia.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Mgea5 in human lymph node using Mgea5 Polyclonal Antibody (Product # PA5-57540) shows moderate to strong cytoplasmic positivity in germinal center cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Mgea5 in human endometrium using Mgea5 Polyclonal Antibody (Product # PA5-57540) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Mgea5 in human skeletal muscle using Mgea5 Polyclonal Antibody (Product # PA5-57540) shows no positivity in myocytes as expected.
