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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ6503 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- O-GlcNAcase Antibody / OGA / MGEA5
- Antibody type
- Polyclonal
- Description
- This highly specific O-GlcNAcase antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the O-GlcNAcase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HEK293, 2) human HeLa, 3) rat brain and 4) mouse brain lysate with O-GlcNAcase antibody. Predicted molecular weight ~103 kDa, routinely observed at ~130 kDa (cytoplasmic form) and ~75 kDa (nuclear form).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human T-47D cells with O-GlcNAcase antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat spleen tissue with O-GlcNAcase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U-87 MG cells with O-GlcNAcase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= O-GlcNAcase antibody.
