PA5-121188

antibody from Invitrogen Antibodies

Targeting: CGAS C6orf150, MB21D1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-121188

Provider

Invitrogen Antibodies

Product name

cGAS Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive test controls include: U-87MG, Mouse testis, Mouse thymus, Rat lung. The target is usually found in the following locations: Cytoplasm, cytosol. Immunogen sequence: EEKCCRKDCL KLMKYLLEQL KERFKDKKHL DKFSSYHVKT AFFHVCTQNP QDSQWDRKDL GLCFDNCVTY FLQCLRTEKL ENYFIPEFNL FSSNLIDKRS K

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.14 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of cGAS in HeLa cells. Samples were incubated with cGAS Polyclonal antibody (Product # PA5-121188) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of C6orf150 in rat lung using a C6orf150 Polyclonal antibody (Product # PA5-121188). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of C6orf150 in rat lung using a C6orf150 Polyclonal antibody (Product # PA5-121188). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of cGAS in U2OS cells. Samples were incubated with cGAS Polyclonal antibody (Product # PA5-121188) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of cGAS in PC-12 cells. Samples were incubated with cGAS Polyclonal antibody (Product # PA5-121188) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of cGAS in NIH/3T3 cells. Samples were incubated with cGAS Polyclonal antibody (Product # PA5-121188) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of cGAS in paraffin-embedded human colon carcinoma. Samples were incubated with cGAS Polyclonal antibody (Product # PA5-121188) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of cGAS in paraffin-embedded human colon. Samples were incubated with cGAS Polyclonal antibody (Product # PA5-121188) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of cGAS in paraffin-embedded human colon carcinoma. Samples were incubated with cGAS Polyclonal antibody (Product # PA5-121188) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of cGAS in paraffin-embedded human colon. Samples were incubated with cGAS Polyclonal antibody (Product # PA5-121188) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.