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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- RQ6412 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- cGAS Antibody / cGAMP Synthase
- Antibody type
- Polyclonal
- Description
- This highly specific cGAS antibody is suitable for use in Western blot/Immunofluorescence/Flow cytometry/Direct ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the cGAS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human A431, 4) rat lung and 5) mouse RAW264.7 cell lysate with cGAS antibody. Predicted molecular weight ~59 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) Caco-2 and 3) A431 cell lysate with cGAS antibody. Predicted molecular weight ~59 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human Caco-2 cells with cGAS antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human ThP-1 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human U937 cells with cGAS antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= cGAS antibody.
