Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-18574 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNAIL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of SNAIL was achieved by transfecting MCF-7 with SNAIL specific siRNAs (Silencer® select Product # s13186, s13187). Western blot analysis (Fig. a) was performed using whole cell extracts from the SNAIL knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with SNAIL Polyclonal Antibody (Product # PA5-18574, 0.2 µg/mL) and Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SNAIL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SNAIL polyclonal Antibody (Product # PA5-18574) and 29 kDa band corresponding to SNAIL was observed across the cell lines tested along with uncharacterized band (*) at ~80 kDa. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), MCF-7 treated LPS (5 µg/mL for 48hrs) (Lane 2), MDA-MB-231 (Lane 3) and HCT 116 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005) (https://doi.org/10.3892/or.2012.2080).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SNAIL in Human Kidney using a SNAIL monoclonal antibody (Product #PA5-18574) at 5 µg/mL. The Human Kidney tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.