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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP2054a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP2054a, RRID:AB_352914
- Product name
- SNAIL Antibody (N-term D24)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1.01 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references FOXM1 Promotes Lung Adenocarcinoma Invasion and Metastasis by Upregulating SNAIL.
MTA3 regulates CGB5 and Snail genes in trophoblast.
Inhibition of p53 induces invasion of serous borderline ovarian tumor cells by accentuating PI3K/Akt-mediated suppression of E-cadherin.
The epithelial mesenchymal transition process in wilms tumor: a study based on a xenograft model.
Pin1 promotes transforming growth factor-beta-induced migration and invasion.
Loss of 15-hydroxyprostaglandin dehydrogenase increases prostaglandin E2 in pancreatic tumors.
Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10.
Up-regulation of alpha5-integrin by E-cadherin loss in hypoxia and its key role in the migration of extravillous trophoblast cells during early implantation.
Anti-invasive activity of histone deacetylase inhibitors via the induction of Egr-1 and the modulation of tight junction-related proteins in human hepatocarcinoma cells.
Aberrant activation of hedgehog signaling pathway in ovarian cancers: effect on prognosis, cell invasion and differentiation.
Small C-terminal domain phosphatase enhances snail activity through dephosphorylation.
Epithelial mesenchymal transition in human ocular chronic graft-versus-host disease.
Beta-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium.
Myofibroblast transdifferentiation of mesothelial cells is mediated by RAGE and contributes to peritoneal fibrosis in uraemia.
Hepatocyte growth factor induces cell scattering through MAPK/Egr-1-mediated upregulation of Snail.
Wei P, Zhang N, Wang Y, Li D, Wang L, Sun X, Shen C, Yang Y, Zhou X, Du X
International journal of biological sciences 2015;11(2):186-98
International journal of biological sciences 2015;11(2):186-98
MTA3 regulates CGB5 and Snail genes in trophoblast.
Chen Y, Miyazaki J, Nishizawa H, Kurahashi H, Leach R, Wang K
Biochemical and biophysical research communications 2013 Apr 19;433(4):379-84
Biochemical and biophysical research communications 2013 Apr 19;433(4):379-84
Inhibition of p53 induces invasion of serous borderline ovarian tumor cells by accentuating PI3K/Akt-mediated suppression of E-cadherin.
Cheng JC, Auersperg N, Leung PC
Oncogene 2011 Mar 3;30(9):1020-31
Oncogene 2011 Mar 3;30(9):1020-31
The epithelial mesenchymal transition process in wilms tumor: a study based on a xenograft model.
Giner F, Machado I, Noguera R, Villamon E, Pellin A, Calabuig-Fariñas S, Peydro-Olaya A, Navarro S, Llombart-Bosch A
Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry 2011 Jul;19(4):369-75
Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry 2011 Jul;19(4):369-75
Pin1 promotes transforming growth factor-beta-induced migration and invasion.
Matsuura I, Chiang KN, Lai CY, He D, Wang G, Ramkumar R, Uchida T, Ryo A, Lu K, Liu F
The Journal of biological chemistry 2010 Jan 15;285(3):1754-64
The Journal of biological chemistry 2010 Jan 15;285(3):1754-64
Loss of 15-hydroxyprostaglandin dehydrogenase increases prostaglandin E2 in pancreatic tumors.
Pham H, Chen M, Li A, King J, Angst E, Dawson DW, Park J, Reber HA, Hines OJ, Eibl G
Pancreas 2010 Apr;39(3):332-9
Pancreas 2010 Apr;39(3):332-9
Human mesenchymal stem cells induce E-cadherin degradation in breast carcinoma spheroids by activating ADAM10.
Dittmer A, Hohlfeld K, Lützkendorf J, Müller LP, Dittmer J
Cellular and molecular life sciences : CMLS 2009 Sep;66(18):3053-65
Cellular and molecular life sciences : CMLS 2009 Sep;66(18):3053-65
Up-regulation of alpha5-integrin by E-cadherin loss in hypoxia and its key role in the migration of extravillous trophoblast cells during early implantation.
Arimoto-Ishida E, Sakata M, Sawada K, Nakayama M, Nishimoto F, Mabuchi S, Takeda T, Yamamoto T, Isobe A, Okamoto Y, Lengyel E, Suehara N, Morishige K, Kimura T
Endocrinology 2009 Sep;150(9):4306-15
Endocrinology 2009 Sep;150(9):4306-15
Anti-invasive activity of histone deacetylase inhibitors via the induction of Egr-1 and the modulation of tight junction-related proteins in human hepatocarcinoma cells.
Kim SO, Choi BT, Choi IW, Cheong J, Kim GY, Kwon TK, Kim ND, Choi YH
BMB reports 2009 Oct 31;42(10):655-60
BMB reports 2009 Oct 31;42(10):655-60
Aberrant activation of hedgehog signaling pathway in ovarian cancers: effect on prognosis, cell invasion and differentiation.
Liao X, Siu MK, Au CW, Wong ES, Chan HY, Ip PP, Ngan HY, Cheung AN
Carcinogenesis 2009 Jan;30(1):131-40
Carcinogenesis 2009 Jan;30(1):131-40
Small C-terminal domain phosphatase enhances snail activity through dephosphorylation.
Wu Y, Evers BM, Zhou BP
The Journal of biological chemistry 2009 Jan 2;284(1):640-8
The Journal of biological chemistry 2009 Jan 2;284(1):640-8
Epithelial mesenchymal transition in human ocular chronic graft-versus-host disease.
Ogawa Y, Shimmura S, Kawakita T, Yoshida S, Kawakami Y, Tsubota K
The American journal of pathology 2009 Dec;175(6):2372-81
The American journal of pathology 2009 Dec;175(6):2372-81
Beta-catenin activation and epithelial-mesenchymal transition in the pathogenesis of pterygium.
Kato N, Shimmura S, Kawakita T, Miyashita H, Ogawa Y, Yoshida S, Higa K, Okano H, Tsubota K
Investigative ophthalmology & visual science 2007 Apr;48(4):1511-7
Investigative ophthalmology & visual science 2007 Apr;48(4):1511-7
Myofibroblast transdifferentiation of mesothelial cells is mediated by RAGE and contributes to peritoneal fibrosis in uraemia.
De Vriese AS, Tilton RG, Mortier S, Lameire NH
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2006 Sep;21(9):2549-55
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association 2006 Sep;21(9):2549-55
Hepatocyte growth factor induces cell scattering through MAPK/Egr-1-mediated upregulation of Snail.
Grotegut S, von Schweinitz D, Christofori G, Lehembre F
The EMBO journal 2006 Aug 9;25(15):3534-45
The EMBO journal 2006 Aug 9;25(15):3534-45
No comments: Submit comment
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of SNAIL (arrow) using rabbit polyclonal hSNAIL-D24 (Cat. #AP2054a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAIL gene (Lane 2).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- HepG2 cells were incubated with HGF for the time periods indicated. LiCl and MG132 were added 8 h before lysis of the cells. Snail protein levels and beta actin as loading control were analyzed by WB.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human Spleen tissue reacted with SNAIL antibody (N-term D24) (Cat.#AP2054a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
