Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [3]
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Validation data
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- Product number
- PA5-11923 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNAIL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Obg-Like ATPase 1 Enhances Chemoresistance of Breast Cancer via Activation of TGF-β/Smad Axis Cascades.
Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro.
Pleiotropic Effects of Epithelial Mesenchymal Crosstalk on Head and Neck Cancer: EMT and beyond.
AurkA controls self-renewal of breast cancer-initiating cells promoting wnt3a stabilization through suppression of miR-128.
A novel vascular pattern promotes metastasis of hepatocellular carcinoma in an epithelial-mesenchymal transition-independent manner.
Liu J, Miao X, Xiao B, Huang J, Tao X, Zhang J, Zhao H, Pan Y, Wang H, Gao G, Xiao GG
Frontiers in pharmacology 2020;11:666
Frontiers in pharmacology 2020;11:666
Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro.
Liu J, Yang Q, Xiao KC, Dobleman T, Hu S, Xiao GG
BMC molecular and cell biology 2020 Sep 14;21(1):65
BMC molecular and cell biology 2020 Sep 14;21(1):65
Pleiotropic Effects of Epithelial Mesenchymal Crosstalk on Head and Neck Cancer: EMT and beyond.
Steinbichler TB, Savic D, Dejaco D, Romani A, Kofler B, Skvortsova II, Riechelmann H, Dudas J
Cancer microenvironment : official journal of the International Cancer Microenvironment Society 2019 Dec;12(2-3):67-76
Cancer microenvironment : official journal of the International Cancer Microenvironment Society 2019 Dec;12(2-3):67-76
AurkA controls self-renewal of breast cancer-initiating cells promoting wnt3a stabilization through suppression of miR-128.
Eterno V, Zambelli A, Villani L, Tuscano A, Manera S, Spitaleri A, Pavesi L, Amato A
Scientific reports 2016 Jun 24;6:28436
Scientific reports 2016 Jun 24;6:28436
A novel vascular pattern promotes metastasis of hepatocellular carcinoma in an epithelial-mesenchymal transition-independent manner.
Fang JH, Zhou HC, Zhang C, Shang LR, Zhang L, Xu J, Zheng L, Yuan Y, Guo RP, Jia WH, Yun JP, Chen MS, Zhang Y, Zhuang SM
Hepatology (Baltimore, Md.) 2015 Aug;62(2):452-65
Hepatology (Baltimore, Md.) 2015 Aug;62(2):452-65
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAIL (arrow) using a SNAIL polyclonal antibody (Product # PA5-11923) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the SNAIL gene (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAIL in HepG2 cells that were incubated with HGF and a SNAIL polyclonal antibody (Product # PA5-11923) for the time periods indicated. LiCl and MG132 were added 8 h before lysis of the cells. Snail protein levels and beta actin as loading control were analyzed by WB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue using a SNAIL polyclonal antibody (Product # PA5-11923), followed by HRP-conjugated secondary antibody and AEC staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Down regulated OLA1 inhibits EMT progress in drug-resistant cell lines. (A) The effect of OLA1 knockdown on expression of Snail, Slug, Vimentin, Zeb1, and MMP9 were evaluated by qRT-PCR. (B) mRNA levels of Snail, Slug, Vimentin, Zeb1, and MMP9 in MCF-7-PTR were calculated comparing with MCF-7. (C) The effect of OLA1-KD on E-cadherin, Vimentin, and Snail 1, Slug, and Zeb1 protein levels were confirmed by western blotting. (D) Protein levels of E-cadherin, Vimentin, and Snail 1, Slug, and Zeb1 in MCF-7-PTR were calculated comparing with MCF-7. (E) mRNA levels of OLA1, Snail, E-cadherin, Vimentin, and Zeb1 in MCF-7-PTR-siOLA1 were calculated comparing with siNC. (* P < 0.05, ** P < 0.01, *** P < 0.001, Student's t-test). ns, no significance.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 OLA1 regulates oral cancer metastasis through Smad2/Smad4 mediated EMT pathway. a mRNA fold change level of EMT markers between UM-1 siR-OLA1 cell and siR-NC cell. mRNA fold change level of EMT marker in UM-2 OLA1 knock down cells ( b ) and UM-1 OLA1 overexpression cells ( c ). d mRNA fold change level of TGFbeta/SMDA2 pathway markers between UM-1 siR-OLA1 cells and siR-NC cells. mRNA fold change level of TGFbeta/SMDA2 pathway markers in UM-2 OLA1 knock down cells ( e ) and UM-1 OLA1 overexpression cells ( f ). g Protein level of EMT marker in UM-1 OLA1 overexpression cells and UM-2 OLA1 knock down cells. h Protein level of TGFbeta/SMDA2 pathway markers in UM-1 OLA1 overexpression cells and UM-2 OLA1 knock down cells. i Western blotting assay showed SMAD2 was silenced by siR-SMAD2 in UM-2 shOLA1 cells. j Western blotting assay showed that silenced SMAD2 decreased OLA1 expression in UM-2 cells. k Wound healing assay showed SMAD2 silenced could rescue enhanced metastasis induced by knocking down OLA1 in UM-2 cells ( n = 3). l Transwell assay to examine the effect of OLA1 on metastasis by smad2 in UM-2 cells ( n = 3). vs. Control, * P < 0.05, ** P < 0.01, *** P < 0.001