Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-23472 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNAIL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial-mesenchymal transition-related markers.
The Vitamin D Analog, MART-10, Attenuates Triple Negative Breast Cancer Cells Metastatic Potential.
MART-10, the vitamin D analog, is a potent drug to inhibit anaplastic thyroid cancer cell metastatic potential.
Li Y, Gong H, Feng L, Mao D, Xiao Y, Wang Y, Huang L
FEBS open bio 2021 Feb;11(2):423-434
FEBS open bio 2021 Feb;11(2):423-434
The Vitamin D Analog, MART-10, Attenuates Triple Negative Breast Cancer Cells Metastatic Potential.
Chiang KC, Yeh TS, Chen SC, Pang JH, Yeh CN, Hsu JT, Chen LW, Kuo SF, Takano M, Kittaka A, Chen TC, Sun CC, Juang HH
International journal of molecular sciences 2016 Apr 21;17(4)
International journal of molecular sciences 2016 Apr 21;17(4)
MART-10, the vitamin D analog, is a potent drug to inhibit anaplastic thyroid cancer cell metastatic potential.
Chiang KC, Kuo SF, Chen CH, Ng S, Lin SF, Yeh CN, Chen LW, Takano M, Chen TC, Juang HH, Kittaka A, Lin JD, Pang JH
Cancer letters 2015 Dec 1;369(1):76-85
Cancer letters 2015 Dec 1;369(1):76-85
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAI1 in human MCF7 cell lysate using 1) prebleed at 1:5000 dilution and 2) a SNAI1/Snail polyclonal antibody (Product # PA5-23472) at 5 µg/mL, and 3) in mouse D3 cell lysate using a SNAI1/Snail polyclonal antibody (Product # PA5-23472) at 3 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAIL in human MCF7 cell lysate using (1) prebleed at 1:5000 dilution and (2) this antibody. Samples were incubated in SNAIL polyclonal antibody (Product # PA5-23472) followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Effects of 1alpha,25(OH) 2 D 3 or MART-10 on the expressions of EMT-related transcriptional factors, Snail, Slug, and Twist, in MDA-MB-231 cells. ( A ) Western blot depicting the expressions of Snail, Slug, and Twist in MDA-MB-231 cells after 10 -7 M 1alpha,25(OH) 2 D 3 or MART-10 treatment. Tubulin was used as the loading control; and ( B - D ) quantitative analysis of Snail, Slug, and Twist expression levels of MDA-MB-231 cells after two days of 10 -7 M 1alpha,25(OH) 2 D 3 or MART-10 treatment. Each value was a mean +- SD of three independent determinations (* p < 0.05, ** p < 0.01).