Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-23482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNAIL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Peritumoral monocytes induce cancer cell autophagy to facilitate the progression of human hepatocellular carcinoma.
Chen DP, Ning WR, Li XF, Wei Y, Lao XM, Wang JC, Wu Y, Zheng L
Autophagy 2018;14(8):1335-1346
Autophagy 2018;14(8):1335-1346
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SNAIL in 0.5 mg/mL MCF-7 lysate. Samples were incubated in SNAIL polyclonal antibody (Product # PA5-23482). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SNAIL in formalin-fixed paraffin-embedded tissue section of mouse placenta. Samples were incubated in SNAIL polyclonal antibody (Product # PA5-23482) using a dilution of 1:200 followed by HRP labeled anti-rabbit secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SNAIL in human kidney tissue. Samples were incubated in SNAIL polyclonal antibody (Product # PA5-23482) using a dilution of 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. The NFKB-SNAI1 pathway mediates the autophagy-enhanced migration of cancer cells. (a) HepG2 cells were stimulated with CCM or TCM for 20 h. The expression levels of SNAI1, SNAI2, TWIST1, and TWIST2 were measured by western blotting. (b-d) HepG2 cells were transfected with shNC, sh ATG5 , or sh ATG7 lentiviral vectors and then treated with CCM or TCM for 20 h (b), 30 min (d), or other time intervals (c). The levels of SNAI1, ATG5, ATG7, p-RELA, RELA, p-AKT, AKT, p-MAPK14, MAPK14, p-MAPK1/3, MAPK1/3, p-MAPK8/9, and MAPK8/9 were determined by western blotting (b and c). Translocation of the RELA protein was analyzed by confocal microscopy (n = 5) (d). (e) HepG2 cells were transfected with control, si- RELA , or si- SNAI1 RNAs before being exposed to CCM or TCM for 20 h, and then their migration abilities were analyzed (n = 6). (f) Sections of hepatoma samples were double stained with anti-human LC3B (green) and anti-human SNAI1 (red) Abs or anti-human LC3B (green) and anti-human RELA (red) Abs. The levels of SNAI1 and nuclear-located RELA expression at the invading edge of human HCCs with high or low LC3B expression were determined by confocal microscopy. Blue, DAPI; n = 5. One out of 5 representative graphs is shown in A-F. The results shown in D, E and F are expressed as the means +- SEM. ** P < 0.01, *** P < 0.001.