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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C99326 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-C99326, RRID:AB_2096969
- Product name
- EEF1A1 Antibody (aa46-72) LS-C99326
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of EEF1A1 Antibody in Y79, T47D cell line lysates (35 ug/lane). EEF1A1 (arrow) was detected using the purified antibody.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- With 293T cell line lysate,resolved proteins were electrophoretically transferred to PVDF membrane and incubated sequentially with primary antibody EF1A1(1:500, 4 degrees C overnight ) and horseradish peroxidase-conjugated second antibody (rabbit). After washing, the bound antibody complex was detected using an ECL chemiluminescence reagent and XAR film (Kodak).
Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain with EEF1A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain with EEF1A1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
