11402-1-AP

antibody from Proteintech Group

Targeting: EEF1A1 EE1A1, EEF1A, EF1A, LENG7

Provider product page for 11402-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [35]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 11402-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#11402-1-AP, RRID:AB_2096966
Product name: EEF1A1 antibody
Antibody type: Polyclonal
Description: KD/KO validated EEF1A1 antibody (Cat. #11402-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

EEF1A1 protein structure - 11402-1-AP shown in red.

Submitted references Active transport of tRNAs facilitates distributed protein synthesis.

Petrosino JM, Courelli V, Uchida K, Bond C, Cooperman B, Bogush A, Lakadamyali M, Prosser BL
bioRxiv : the preprint server for biology 2026 Jan 27;

Regulation of UBC12 expression and protein neddylation by PINK1 suggests a primate-specific function.

Huang W, Xu T, Zhou GK, Li YX, Lin CZ, Chen XS, Zhang T, Jiang L, Liu YT, Xiong X, Liang XY, Guo XY, Li SH, Li XJ, Yang WL
Zoological research 2025 Nov 18;46(6):1488-1500

Targeting TUBB2B inhibits triple-negative breast cancer growth and brain-metastatic colonization.

He Q, Hu J, Ngo FY, Zhang H, He L, Huang H, Wu T, Pan Y, Yang Z, Jiang Y, Cho WC, Cheuk W, Tse GM, Tsang JY, Yang M, Zhang L, Wang X, Lo PC, Lau CG, Chin YR
Journal of experimental & clinical cancer research : CR 2025 Feb 17;44(1):55

Eukaryotic translation elongation factor 1 alpha 1 facilitates coxsackievirus B replication through interacting with PABP, viral protein 3 CD and viral RNA.

Zhao S, Dong Y, Wang Y, Yang H, Luan T, He Y, Lin L, Chen Y, Fei Y, Wang G, Wang Y, Zhong Z, Qi N, Zhao W
Virulence 2025 Dec;16(1):2563925

N6-methyladenosine triggers renal fibrosis via enhancing translation and stability of ZEB2 mRNA.

Cai Y, Zhou J, Xu A, Huang J, Zhang H, Xie G, Zhong K, Wu Y, Ye P, Wang H, Niu H
The Journal of biological chemistry 2024 Sep;300(9):107598

miR-31-mediated local translation at the mitotic spindle is important for early development.

Remsburg CM, Konrad KD, Testa MD, Stepicheva N, Lee K, Choe LH, Polson S, Bhavsar J, Huang H, Song JL
Development (Cambridge, England) 2024 Sep 1;151(17)

Temporal alterations of the nascent proteome in response to mitochondrial stress.

Stępkowski TM, Linke V, Stadnik D, Zakrzewski M, Zawada AE, Serwa RA, Chacinska A
Cell reports 2024 Oct 22;43(10):114803

MILIP Binding to tRNAs Promotes Protein Synthesis to Drive Triple-Negative Breast Cancer.

Zheng SM, Feng YC, Zhu Q, Li RQ, Yan QQ, Teng L, Yue YM, Han MM, Ye K, Zhang SN, Qi TF, Tang CX, Zhao XH, Zhang YY, Xu L, Xu R, Xing J, Baker M, Liu T, Thorne RF, Jin L, Preiss T, Zhang XD, Cang S, Gao JN
Cancer research 2024 May 2;84(9):1460-1474

Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK.

Li Y, Munoz-Mayorga D, Nie Y, Kang N, Tao Y, Lagerwall J, Pernaci C, Curtin G, Coufal NG, Mertens J, Shi L, Chen X
Cell metabolism 2024 Jun 4;36(6):1351-1370.e8

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice.

Zou D, Li K, Su L, Liu J, Lu Y, Huang R, Li M, Mang X, Geng Q, Li P, Tang J, Yu Z, Zhang Z, Chen D, Miao S, Yu J, Yan W, Song W
Developmental cell 2024 Jul 8;59(13):1707-1723.e8

A single-cell atlas of lung homeostasis reveals dynamic changes during development and aging.

Jia H, Chang Y, Chen Y, Chen X, Zhang H, Hua X, Xu M, Sheng Y, Zhang N, Cui H, Han L, Zhang J, Fu X, Song J
Communications biology 2024 Apr 8;7(1):427

CRISPR Screen Identifies the RNA-Binding Protein Eef1a1 as a Key Regulator of Myogenesis.

Liu W, Wang W, Wang Z, Fan X, Li W, Huang Y, Yang X, Tang Z
International journal of molecular sciences 2024 Apr 28;25(9)

Valbenazine promotes body growth via growth hormone signaling during zebrafish embryonic development.

Su Z, Dai Z, Qin F, Zhang H, Zheng M, Zhu Y, Tong Z, Song W, Li X
Toxicology and applied pharmacology 2023 Oct 15;477:116674

Gelation of cytoplasmic expanded CAG RNA repeats suppresses global protein synthesis.

Pan Y, Lu J, Feng X, Lu S, Yang Y, Yang G, Tan S, Wang L, Li P, Luo S, Lu B
Nature chemical biology 2023 Nov;19(11):1372-1383

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate.

Zhou P, Chang WY, Gong DA, Xia J, Chen W, Huang LY, Liu R, Liu Y, Chen C, Wang K, Tang N, Huang AL
Cell metabolism 2023 Nov 7;35(11):1961-1975.e6

miR-31-mediated local translation at the mitotic spindle is important for early development.

Remsburg CM, Konrad K, Stepicheva N, Testa M, Lee K, Choe L, Polson S, Bhavsar J, Huang H, Song JL
Research square 2023 Jun 14;

Anisomycin inhibits Coxsackievirus B replication by promoting the lysosomal degradation of eEF1A1.

Shao E, Zhao S, Dong Y, Wang Y, Fei Y, Li S, Wang L, Bashir T, Luan T, Lin L, Wang Y, Zhao W, Zhong Z
Antiviral research 2023 Jul;215:105621

Immunoproteasome-specific subunit PSMB9 induction is required to regulate cellular proteostasis upon mitochondrial dysfunction.

Kim M, Serwa RA, Samluk L, Suppanz I, Kodroń A, Stępkowski TM, Elancheliyan P, Tsegaye B, Oeljeklaus S, Wasilewski M, Warscheid B, Chacinska A
Nature communications 2023 Jul 11;14(1):4092

Epitranscriptic regulation of HRAS by N(6)-methyladenosine drives tumor progression.

Pan Y, Gu Y, Liu T, Zhang Q, Yang F, Duan L, Cheng S, Zhu X, Xi Y, Chang X, Ye Q, Gao S
Proceedings of the National Academy of Sciences of the United States of America 2023 Apr 4;120(14):e2302291120

A photoaffinity labeling strategy identified EF1A1 as a binding protein of cyclic dinucleotide 2'3'-cGAMP.

Hou Y, Lu H, Li J, Guan Z, Zhang J, Zhang W, Yin C, Sun L, Zhang Y, Jiang H
Cell chemical biology 2022 Jan 20;29(1):133-144.e20

N(6)-methyladenosine modification regulates imatinib resistance of gastrointestinal stromal tumor by enhancing the expression of multidrug transporter MRP1.

Xu K, Zhang Q, Chen M, Li B, Wang N, Li C, Gao Z, Zhang D, Yang L, Xu Z, Li X, Xu H
Cancer letters 2022 Apr 1;530:85-99

CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.

Asamitsu S, Yabuki Y, Ikenoshita S, Kawakubo K, Kawasaki M, Usuki S, Nakayama Y, Adachi K, Kugoh H, Ishii K, Matsuura T, Nanba E, Sugiyama H, Fukunaga K, Shioda N
Science advances 2021 Jan;7(3)

Cytosolic PINK1 orchestrates protein translation during proteasomal stress by phosphorylating the translation elongation factor eEF1A1.

Qin S, Ye L, Zheng Y, Gao J
FEBS letters 2021 Feb;595(4):507-520

Opposing roles of C/EBPα and eEF1A1 in Sp1-regulated miR-122 transcription.

Zeng C, Sang Y, Wang FY, Zhuang SM
RNA biology 2020 Feb;17(2):202-210

A rapid and specific method to simultaneously quantify eukaryotic elongation factor 1A1 and A2 protein levels in cancer cells.

Bosutti A, Kalaja O, Zanconati F, Dapas B, Grassi G, Passamonti S, Scaggiante B
Journal of pharmaceutical and biomedical analysis 2019 Nov 30;176:112814

RNA m(6)A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail.

Lin X, Chai G, Wu Y, Li J, Chen F, Liu J, Luo G, Tauler J, Du J, Lin S, He C, Wang H
Nature communications 2019 May 6;10(1):2065

Novel nannocystin A analogues as anticancer therapeutics: Synthesis, biological evaluations and structure-activity relationship studies.

Liu Q, Yang X, Ji J, Zhang SL, He Y
European journal of medicinal chemistry 2019 May 15;170:99-111

Accumulation of EBI3 induced by virulent Mycobacterium tuberculosis inhibits apoptosis in murine macrophages.

Deng JH, Chen HY, Huang C, Yan JM, Yin Z, Zhang XL, Pan Q
Pathogens and disease 2019 Feb 1;77(1)

Contradictory mRNA and protein misexpression of EEF1A1 in ductal breast carcinoma due to cell cycle regulation and cellular stress.

Lin CY, Beattie A, Baradaran B, Dray E, Duijf PHG
Scientific reports 2018 Sep 17;8(1):13904

Acetylation of Cytidine in mRNA Promotes Translation Efficiency.

Arango D, Sturgill D, Alhusaini N, Dillman AA, Sweet TJ, Hanson G, Hosogane M, Sinclair WR, Nanan KK, Mandler MD, Fox SD, Zengeya TT, Andresson T, Meier JL, Coller J, Oberdoerffer S
Cell 2018 Dec 13;175(7):1872-1886.e24

Single-cell gene expression analysis reveals diversity among human spermatogonia.

Neuhaus N, Yoon J, Terwort N, Kliesch S, Seggewiss J, Huge A, Voss R, Schlatt S, Grindberg RV, Schöler HR
Molecular human reproduction 2017 Feb 10;23(2):79-90

The Ubiquitin-like Protein FAT10 Stabilizes eEF1A1 Expression to Promote Tumor Proliferation in a Complex Manner.

Liu X, Chen L, Ge J, Yan C, Huang Z, Hu J, Wen C, Li M, Huang D, Qiu Y, Hao H, Yuan R, Lei J, Yu X, Shao J
Cancer research 2016 Aug 15;76(16):4897-907

Bioinformatics analysis of proteomics profiles in senescent human primary proximal tubule epithelial cells.

Lu Y, Wang J, Dapeng C, Wu D, Cai G, Chen X
BMC nephrology 2016 Apr 1;17:39

Identification of differentially expressed genes in human cryptorchid testes using suppression subtractive hybridization.

Mizuno K, Kojima Y, Kurokawa S, Maruyama T, Sasaki S, Kohri K, Hayashi Y
The Journal of urology 2009 Mar;181(3):1330-7; discussion 1337

Quantitative proteomic analysis of a paired human liver healthy versus carcinoma cell lines with the same genetic background to identify potential hepatocellular carcinoma markers.

Bao H, Song P, Liu Q, Liu Y, Yun D, Saiyin H, Du R, Zhang Y, Fan H, Yang P, Chen X
Proteomics. Clinical applications 2009 Jun;3(6):705-19

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 11402-1-AP(EEF1A1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using EEF1A1 antibody 11402-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas cancer using 11402-1-AP(EEF1A1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human pancreas cancer using 11402-1-AP(EEF1A1 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue