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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34709 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEF1A1 Recombinant Rabbit Monoclonal Antibody (JB44-13)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Rat brain tissue, mouse skeletal muscle tissue lysate, rat skin tissue lysate, Daudi, mouse cerebellum tissue lysate, HepG2, HUVEC, SH-SY-5Y, rat skeletal muscle tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse smooth muscle tissue, THP-1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB44-13
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of eEF1A1 in Lane 1: Rat brain tissue, Lane 2: Mouse skeletal muscle, Lane 3: Mouse cerebellum, Lane 4: Rat skin, Lane 5: Daudi . Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of eEF1A1 in SH-SY-5Y cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of eEF1A1 in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of eEF1A1 in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with eEF1A1 monoclonal antibody (Product # MA5-34709), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of eEF1A1 in THP-1 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with eEF1A1 monoclonal antibody (Product # MA5-34709) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
